PMID: 6985146Dec 1, 1982Paper

Ca2+-dependent phosphorylation and Ca2+ uptake in membrane fractions of the mesenteric artery

Journal of Muscle Research and Cell Motility
S Thorens

Abstract

Ca2+-dependent phosphorylation of endogenous substrate proteins (mol. wt 30 800, 35 500, 38 600 and 53 200) is found in a membrane subcellular fraction from rabbit mesenteric arteries. Characteristics of 32P incorporation are suggestive of a phosphoester-type phosphorylation produced by a Ca2+-dependent protein kinase. Ca2+-dependent phosphorylation and Ca2+ uptake rate show comparable affinities for Ca2+ of 3.5 x 10(-7) M and 2.4 x 10(-7) M, respectively. The dependence of both phenomena on the MgATP concentration is also similar. Ca2+-dependent phosphorylation and Ca2+ uptake are inhibited by trifluoperazine with an IC50 of 3 x 10(-5) M and 5 x 10(-5) M, respectively. These results suggest that Ca2+ uptake might be modulated by a Ca2+-dependent protein kinase, which is possibly regulated by membrane-bound calmodulin. Endogenous Ca2+-dependent phosphorylation is stimulated up to 300% by the addition of boiled cytosol. This stimulation is due to phosphorylation of proteins of molecular weight 21 000 and 81 500 and is reversed by trifluoperazine. Since this stimulation cannot be mimicked by addition of calmodulin or phosphatidylserine, and since boiled cytosol does not stimulate Ca2+ uptake, it is proposed that an unknown cytoso...Continue Reading

References

Oct 24, 1977·Biochemical and Biophysical Research Communications·R DabrowskaD J Hartshorne
Jan 1, 1977·The American Journal of Physiology·R A JanisE E Daniel
Jul 18, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·D F Fitzpatrick, A Szentivanyi
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Schulman, P Greengard
Jul 1, 1979·Journal of Molecular and Cellular Cardiology·C Y KwanE E Daniel
Feb 16, 1972·Biochemical and Biophysical Research Communications·J H WangT H Wang
Apr 21, 1972·Science·D F FitzpatrickL Hurwitz
Feb 12, 1981·Biochemical and Biophysical Research Communications·R C SchatzmanJ F Kuo
Jun 9, 1981·Biochimica Et Biophysica Acta·N MorelT Godfraind

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Citations

Jun 19, 1986·Biochimica Et Biophysica Acta·L Raeymaekers, L R Jones

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