PMID: 9655599Jul 9, 1998Paper

Ca2+-H+ antiport activity in synaptic vesicles isolated from sheep brain cortex

Neuroscience Letters
Paula P GonçalvesM G Vale

Abstract

Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. This transport activity was sensitive to the V-type ATPase inhibitor, bafilomycin, but not to the P-type ATPase inhibitor, vanadate. We also observed that the proton gradient, established across the synaptic vesicle membranes in the presence of ATP, is partially dissipated by the addition of Ca2+ (100-860 microM) in correlation to an increase of ATP hydrolysis by the H+-pumping ATPase. In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. These results indicate that synaptic vesicles accumulate Ca2+, via a low affinity Ca2+-H+ antiport system energized by the protonmotive force originated from the H+-pumping ATPase activity.

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Citations

Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O H PetersenA V Tepikin
Apr 27, 1999·Brain Research. Molecular Brain Research·P P GonçalvesM G Vale
Oct 16, 1999·Brain Research. Molecular Brain Research·P P GonçalvesM G Vale
Jun 24, 2000·Biochimie·Y Dunant, M Israël
Mar 17, 2000·Brain Research. Brain Research Protocols·P P GonçalvesM G Vale
Feb 24, 2016·Frontiers in Synaptic Neuroscience·Shigeo Takamori
Oct 28, 2006·Cell Calcium·Martin OheimWalter Stühmer
Nov 1, 2008·The European Journal of Neuroscience·Jaime SantodomingoJavier Alvarez
Apr 24, 2013·Journal of Neurochemistry·Joao Miguel CordeiroYves Dunant
May 3, 2005·Cell Calcium·Alfredo MorenoJavier Alvarez
Jan 24, 2009·Annals of the New York Academy of Sciences·Yves DunantPaula P Gonçalves
Nov 10, 2010·The Journal of Physiology·J Miguel CordeiroYves Dunant
Aug 28, 2010·The European Journal of Neuroscience·Satoko Soga-SakakibaraKenji Kuba
Jan 10, 2012·Cell Calcium·Ricardo BorgesJosé David Machado
Apr 11, 2000·Microscopy Research and Technique·Y Dunant
Aug 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yves Dunant, Victor Gisiger
Mar 29, 2000·The Journal of Biological Chemistry·M L MundorfR M Wightman

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