PMID: 8607996Apr 1, 1996Paper

Ca2+ imaging of CNS axons in culture indicates reliable coupling between single action potentials and distal functional release sites

Neuron
P J MackenzieT H Murphy

Abstract

A combination of Ca2+ imaging and current clamp recording in cultured cortical neurons was used to evaluate the reliability of coupling between the action potential and rises in Ca2+ at distal release sites as a possible source of variability in CNS synaptic transmission. Local domains of enhanced Ca2+ influx were observed at varicosities on axon collaterals. Functional assay of vesicle turnover using FM1-43 and parallel electron microscopy confirmed that these varicosities were release sites. Single action potentials reliably ( > 95% of the time) resulted in a presynaptic Ca2+ transient at all presumed release sites including those on distal collaterals. Variability in the amplitude of presynaptic Ca2+ transients at individual boutons was estimated to be on average less than 20%. We conclude that the coupling of somatic action potentials to distal release sites is generally a reliable process, although nonlinearity in the relationship between Ca2+ influx and neurotransmitter release may amplify the effects of relatively small fluctuations in Ca2+ influx.

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Citations

Mar 17, 2004·Molecular and Cellular Neurosciences·Shengxiang Zhang, Timothy H Murphy
Nov 10, 2011·Nature Communications·Arjuna RatnayakaKevin Staras
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rossella Conti, John Lisman
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Mar 18, 2003·The Journal of Physiology·Morten Raastad, Gordon M G Shepherd
Mar 18, 2003·The Journal of Physiology·Dominique Debanne, Michaël Russier
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Feb 19, 2002·The Journal of Physiology·Zhong-Ping FengNaweed I Syed
Jun 27, 2012·The Journal of Physiology·Takuya SasakiYuji Ikegaya
Aug 5, 1999·The Journal of Physiology·K JensenJ D Lambert
Aug 21, 2004·The Journal of Physiology·A F SolengM Raastad
Jan 9, 2007·Muscle & Nerve·Nicolae PetrescuPeter K Stys
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Mar 23, 2004·Nature Reviews. Neuroscience·Dominique Debanne
Jul 13, 2006·Journal of Neurophysiology·Chuan-Li ZhangShing Yan Chiu
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Apr 30, 2011·Physiological Reviews·Dominique DebanneGisèle Alcaraz
Oct 5, 2002·Journal of Neurophysiology·Sergei Kirischuk, Rosemarie Grantyn

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