PMID: 9172074Jun 1, 1997Paper

Ca(2+)-induced conformational shift of the COOH-domain of eel skeletal muscle troponin C in the presence of physiological concentrations of Mg2+

Journal of Muscle Research and Cell Motility
J M FrançoisC Gerday

Abstract

The spectroscopic properties of Trp152 of eel skeletal muscle troponin C have been studied under conditions in which the COOH-domain is depleted of metal ions, titrated with Mg2+ and subsequently with Ca2+. This spectroscopic study clearly shows that the Mg2+ or Ca(2+)-bound states substitution lead to distinct conformations of the COOH-domain. The analysis of eel troponin C absorption and Trp152 fluorescence emission spectra indicates a more polar environment in the Mg(2+)-bound state of the protein. Steady state tryptophan polarization and lifetime distribution data indicate that the motion of the indole moiety is more restricted in the Mg2+ state of the protein than in the Ca(2+)-bound state. However, fluorescence quenching data using I- and Cs+ show that Trp152 is more accessible to the solvent in the Mg(2+)-bound state of eel troponin C. This spectroscopic analysis of the distinct Ca2+ and Mg(2+)-bound states of eel troponin C is consistent with the description of the three-dimensional structure of the corresponding states of pike (pI = 4.10) parvalbumin which is structurally homologous to the COOH-domain of troponin C. Since it appears that during muscular contraction, magnesium ions, which occupy the binding sites of the...Continue Reading

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