Ca(2+) influx through the osteoclastic plasma membrane ryanodine receptor

American Journal of Physiology. Renal Physiology
Baljit S MoongaMone Zaidi

Abstract

We predict that the type 2 ryanodine receptor isoform (RyR-2) located in the osteoclastic membrane functions as a Ca(2+) influx channel and as a divalent cation (Ca(2+)) sensor. Cytosolic Ca(2+) measurements revealed Ca(2+) influx in osteoclasts at depolarized membrane potentials. The cytosolic Ca(2+) change was, as expected, not seen in Ca(2+)-free medium and was blocked by the RyR modulator ryanodine. In contrast, at basal membrane potentials (approximately 25 mV) ryanodine triggered extracellular Ca(2+) influx that was blocked by Ni(2+). In parallel, single-channel recordings obtained from inside-out excised patches revealed a divalent cation-selective approximately 60-pS conductance in symmetric solutions of Ba-aspartate [Ba-Asp; reversal potential (E(rev)) approximately 0 mV]. In the presence of a Ba(2+) gradient, i.e., with Ba-Asp in the pipette and Na-Asp in the bath, channel conductance increased to approximately 120 pS and E(rev) shifted to 21 mV. The conductance was tentatively classified as a RyR-gated Ca(2+) channel as it displayed characteristic metastable states and was sensitive to ruthenium red and a specific anti-RyR antibody, Ab(34). To demonstrate that extracellular Ca(2+) sensing occurred at the osteoclastic...Continue Reading

References

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Citations

May 28, 2014·Neuroscience Letters·Regina JakobShey-Shing Sheu
Aug 11, 2007·Journal of Cell Science·Beatrice B YaroslavskiyHarry C Blair
Nov 23, 2006·Reviews in Endocrine & Metabolic Disorders·Harry C Blair, Mone Zaidi
Jul 17, 2007·Biochemical and Biophysical Research Communications·Rasmus H NielsenKim Henriksen
Mar 10, 2004·Biological Reviews of the Cambridge Philosophical Society·Mone ZaidiChristopher L H Huang
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mone ZaidiChristopher L H Huang
Aug 20, 2008·Biological Reviews of the Cambridge Philosophical Society·Harry C BlairLi Sun
Aug 8, 2006·Biochemical and Biophysical Research Communications·F MocciaLuigia Santella
Jul 10, 2007·Nature Medicine·Mone Zaidi
Sep 17, 2009·Immunological Reviews·Takako Negishi-Koga, Hiroshi Takayanagi
Apr 16, 2010·Annals of the New York Academy of Sciences·Lisa J RobinsonChristopher L-H Huang
Sep 15, 2006·American Journal of Physiology. Cell Physiology·Mikiko ItoKen-ichi Miyamoto
May 8, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Yu-An ZhangHui Zou
Oct 14, 2011·Journal of Muscle Research and Cell Motility·Christopher L-H HuangJames A Fraser
Jun 16, 2011·BioFactors·Harry C BlairMone Zaidi
Dec 13, 2016·Pflügers Archiv : European journal of physiology·Giuseppe AlbanoDaniel G Fuster
Mar 16, 2021·Frontiers in Cell and Developmental Biology·Amy B P RibetNathan J Pavlos
Feb 8, 2005·Biochemical and Biophysical Research Communications·Harry C BlairMone Zaidi
Feb 2, 2008·Archives of Biochemistry and Biophysics·Andrea Del FattoreNadia Rucci

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