PMID: 6106502May 8, 1980Paper

(Ca2+ + Mg2+)-ATPase in enriched sarcolemma from dog heart

Biochimica Et Biophysica Acta
N C Morcos, G I Drummond

Abstract

An enriched fraction of plasma membranes was prepared from canine ventricle by a process which involved thorough disruption of membranes by vigorous homogenization in dilute suspension, sedimentation of contractile proteins and mitochondria at 3000 X g followed by sedimentation of a microsomal fraction at 200 000 X g. The microsomal suspension was then fractionated on a discontinuous sucrose gradient. Particles migrating in the density range 1.0591--1.1083 were characterized by (Na+ + K+)-ATPase activity and [3H]ouabain binding as being enriched in sarcolemma and were comprised of nonaggregated vesicles of diameter approx. 0.1 micron. These fractions contained (Ca2+ + Mg2+)-ATPase which appreared endogenous to the sarcolemma. The enzyme was solubilized using Triton X-100 and 1 M KCl and partially purified. Optimal Ca2+ concentration for enzyme activity was 5--10 microM. Both Na+ and K+ stimulated enzyme activity. It is suggested that the enzyme may be involved in the outward pumping of Ca2+ from the cardiac cell.

References

Sep 7, 1976·Biochimica Et Biophysica Acta·D O LevitskyE I Chazov
Nov 1, 1976·Canadian Journal of Biochemistry·P J St Louis, P V Sulakhe
Apr 21, 1975·Biochimica Et Biophysica Acta·G Meissner
Oct 1, 1976·Archives of Biochemistry and Biophysics·A H CaswellJ P Brunschwig
Jan 1, 1978·Canadian Journal of Biochemistry·N C Morcos, A L Jacobson
Jan 1, 1972·Annual Review of Biochemistry·G Guidotti
Nov 1, 1971·Biochemical and Biophysical Research Communications·A M KidwaiE E Daniel
Jan 1, 1974·Annals of the New York Academy of Sciences·G A Langer, S D Serena
Feb 1, 1969·The Journal of Physiology·P F BakerR A Steinhardt

❮ Previous
Next ❯

Citations

Jan 1, 1982·The Journal of Membrane Biology·P Aaronson, C Van Breemen
Jan 1, 1982·The International Journal of Biochemistry·V PanagiaN S Dhalla
Feb 1, 1985·Cell Differentiation·N M Troccoli, R E Hausman
Jul 17, 1996·Mechanisms of Ageing and Development·N OzakiS Ishibashi
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R E HausmanW A Rosenkrans
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H Gesser, E Jørgensen
Jun 1, 1982·The Journal of Cell Biology·A O JorgensenD H MacLennan
May 15, 1989·Archives of Biochemistry and Biophysics·E QuistP Powell
Dec 15, 1992·Biochemical and Biophysical Research Communications·T KolterJ Eckel
Jun 16, 1981·Biochemical and Biophysical Research Communications·B S TuanaN S Dhalla
Dec 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shinobu NishitaniIchiro Sonaka
Jul 19, 2008·Health Economics, Policy, and Law·Stephen Birch, Amiram Gafni
Mar 10, 2015·Health Economics, Policy, and Law·Stephen Birch, Amiram Gafni
Feb 1, 1983·Biochimica Et Biophysica Acta·J G Church, A K Sen
Feb 1, 1983·Biochimica Et Biophysica Acta·V V KupriyanovV A Saks

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.