PMID: 7525605Nov 1, 1994Paper

Ca2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells

The Journal of Cell Biology
C Erxleben, H Plattner

Abstract

A correlated electrophysiological and light microscopic evaluation of trichocyst exocytosis was carried out the Paramecium cells which possess extensive cortical Ca stores with footlike links to the plasmalemma. We used not only intra- but also extracellular recordings to account for polar arrangement of ion channels (while trichocysts can be released from all over the cell surface). With three widely different secretagogues, aminoethyldextran (AED), veratridine and caffeine, similar anterior Nain and posterior Kout currents (both known to be Ca(2+)-dependent) were observed. Direct de- or hyperpolarization induced by current injection failed to trigger exocytosis. For both, exocytotic membrane fusion and secretagogue-induced membrane currents, sensitivity to or availability of Ca2+ appears to be different. Current responses to AED were blocked by W7 or trifluoperazine, while exocytosis remained unaffected. Reducing [Ca2+]o to < or = 0.16 microM (i.e., resting [Ca2+]i) suppressed electrical membrane responses triggered with AED, while we had previously documented normal exocytotic membrane fusion. From this we conclude that the primary effect of AED (as of caffeine) is the mobilization of Ca2+ from the subplasmalemmal pools whic...Continue Reading

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Citations

Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Helmut Plattner
Jun 24, 2000·Biochimie·L VayssiéJ Cohen
Apr 22, 2009·Molecular and Cellular Biology·Eva-Maria LadenburgerHelmut Plattner
Dec 3, 2013·European Journal of Pharmacology·Roberto DocampoHelmut Plattner
Mar 3, 2007·Trends in Cell Biology·Nels C EldeAaron P Turkewitz
Mar 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Enrico CrivellatoDomenico Ribatti
Sep 5, 2013·The Journal of Eukaryotic Microbiology·Helmut Plattner
Jun 22, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H PlattnerL Bachmann
Jul 19, 2017·The Journal of Eukaryotic Microbiology·Helmut Plattner
Feb 1, 1999·Photochemistry and Photobiology·Hanna FabczakStanistaw Fabcza
Feb 27, 2003·The Journal of Experimental Biology·Yoshiaki Iwadate
Dec 30, 2008·The Journal of Experimental Biology·Yasuo NakaokaNoboru Hashimoto

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