Ca2+ responses to acetylcholine and adenosine triphosphate in the otocyst of chick embryo

Journal of Neurobiology
Y Nakaoka, M Yamashita

Abstract

The action of acetylcholine and adenosine triphosphate (ATP) on cytoplasmic Ca2+ concentration ([Ca2+]i) was studied in the otocyst epithelium of embryonic day 3 chicks with Ca(2+)-sensitive fluorescence measurements. Increases in [Ca2+]i were evoked by the bath application of acetylcholine (1 microM or higher). The rise in [Ca2+]i was due to the release of Ca2+ from intracellular Ca2+ stores, since the Ca2+ response occurred even in a Ca(2+)-free medium. The Ca2+ response to acetylcholine was mediated by muscarinic receptors. Atropine of 1 microM abolished the response to 10 microM acetylcholine; muscarine and carbamylcholine (100 microM each) evoked Ca2+ rises. Increases in [Ca2+]i were also evoked by the bath application of ATP (10 microM or higher). The Ca2+ rise by ATP was evoked even in a Ca(2+)-free medium. Adenosine (500 microM) did not cause any Ca2+ response. Suramin and reactive blue 2 (200 microM each) completely blocked the Ca2+ response to 500 microM ATP. Uridine triphosphate (500 microM) caused comparable Ca2+ responses with those to 500 microM ATP. These results suggested the involvement of P2U purinoceptors. The potentiation of Ca2+ rise was observed when acetylcholine and ATP were co-applied at submaximal conc...Continue Reading

References

Apr 22, 1992·Proceedings. Biological Sciences·P A Fuchs, B W Murrow
Oct 1, 1992·Journal of Neurobiology·C S von BartheldE W Rubel
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·G R Dubyak
Jan 1, 1992·Experimental Neurology·J T Corwin
Jan 1, 1990·Annals of the New York Academy of Sciences·G Burnstock
Apr 1, 1991·Trends in Pharmacological Sciences·S E O'ConnorP Leff
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·E C HulmeN J Buckley
Feb 1, 1990·Developmental Biology·G J SwansonJ Lewis
Apr 1, 1989·Trends in Neurosciences·T I Bonner
Jan 1, 1971·Neuroscience Research·G Filogamo, P C Marchisio
Oct 1, 1994·Trends in Neurosciences·H Zimmermann
Sep 1, 1994·Journal of Neurobiology·M YamashitaY Fukuda
Jan 1, 1994·The Japanese Journal of Physiology·T WakatsukiM Yamamoto
Mar 1, 1994·Trends in Pharmacological Sciences·E A BarnardT E Webb
Dec 1, 1993·Journal of Neurobiology·M Yamashita, Y Fukuda
Jan 28, 1993·Nature·M J Berridge

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Citations

Oct 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Y SakakiM Yamashita
Apr 1, 1997·British Journal of Pharmacology·M van der GietW Zidek
Jul 20, 2000·Clinical and Experimental Pharmacology & Physiology·G D Housley
Jan 12, 2011·Cellular and Molecular Life Sciences : CMLS·Geoffrey Burnstock, Henning Ulrich
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
May 9, 1997·The Journal of Biological Chemistry·Y D BogdanovG Burnstock
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Masayuki Yamashita, Miho Sugioka

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