PMID: 9529322May 16, 1998Paper

Ca2+ signaling modulates cytolytic T lymphocyte effector functions

The Journal of Experimental Medicine
M T EsserV L Braciale

Abstract

Cytolytic T cells use two mechanisms to kill virally infected cells, tumor cells, or other potentially autoreactive T cells in short-term in vitro assays. The perforin/granule exocytosis mechanism uses preformed cytolytic granules that are delivered to the target cell to induce apoptosis and eventual lysis. FasL/Fas (CD95 ligand/CD95)-mediated cytolysis requires de novo protein synthesis of FasL by the CTL and the presence of the death receptor Fas on the target cell to induce apoptosis. Using a CD8(+) CTL clone that kills via both the perforin/granule exocytosis and FasL/Fas mechanisms, and a clone that kills via the FasL/Fas mechanism only, we have examined the requirement of intra- and extracellular Ca2+ in TCR-triggered cytolytic effector function. These two clones, a panel of Ca2+ antagonists, and agonists were used to determine that a large biphasic increase in intracellular calcium concentration, characterized by release of Ca2+ from intracellular stores followed by a sustained influx of extracellular Ca2+, is required for perforin/granule exocytosis. Only the sustained influx of extracellular Ca2+ is required for FasL induction and killing. Thapsigargin, at low concentrations, induces this small but sustained increase i...Continue Reading

References

Jan 1, 1992·Advances in Immunology·H YagitaK Okumura
Sep 1, 1987·The Journal of Experimental Medicine·H Takayama, M V Sitkovsky
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T J BracialeV L Braciale
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Jan 1, 1988·Annals of the New York Academy of Sciences·V H EngelhardL S Gray
Dec 1, 1987·The Journal of Experimental Medicine·J D YoungZ A Cohn
Apr 1, 1981·The Journal of Experimental Medicine·T J BracialeV L Braciale
Aug 1, 1995·Immunological Reviews·C C LiuJ D Young
Jan 1, 1995·International Reviews of Immunology·W R ClarkM Matloubian
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·S T JuA Marshak-Rothstein
Feb 1, 1995·The Journal of Experimental Medicine·F VignauxP Golstein
Mar 10, 1995·Science·S Nagata, P Golstein
Apr 7, 1995·Cell·G Berke
Jun 1, 1995·Current Opinion in Immunology·J H Russell
Jan 1, 1995·Annual Review of Immunology·R S Lewis, M D Cahalan
Jan 1, 1993·The Journal of Experimental Medicine·E RouvierP Golstein
Jan 1, 1995·Advances in Second Messenger and Phosphoprotein Research·M E Cardenas, J Heitman
Mar 1, 1995·Immunology Today·M Izquierdo PastorD Cantrell
Nov 1, 1994·British Journal of Pharmacology·S C ChungP Gardner
Jan 1, 1994·Annual Review of Biochemistry·G R Crabtree, N A Clipstone
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·P A NegulescuM D Cahalan
Nov 1, 1993·The American Journal of Physiology·Z WangL J Mordan
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Zweifach, R S Lewis
Feb 1, 1993·Molecular Biology of the Cell·D M Haverstick, L S Gray
Dec 1, 1995·European Journal of Immunology·A AnelA M Schmitt-Verhulst
Dec 1, 1995·Immunology Today·D H LynchM R Alderson
May 1, 1996·Immunity·P A NegulescuM D Cahalan

❮ Previous
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Citations

Aug 7, 1999·International Journal of Clinical & Laboratory Research·J R NoferM Tepel
May 4, 2001·Journal of Immunological Methods·C BranderJ D Seebach
Jun 29, 2002·Current Opinion in Immunology·Judy LiebermanPremlata Shankar
Dec 17, 2005·Nature Immunology·Elena M GalloGerald R Crabtree
Jun 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Didi MatzaRichard A Flavell
Jan 13, 2006·Immunology and Cell Biology·Ilia Voskoboinik, Joseph A Trapani
May 9, 2012·Molecular and Cellular Biology·Chih-wen Ou-YangWeiguo Zhang
Jun 3, 2000·Annual Review of Immunology·K M MurphyT L Murphy
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mustapha FaroudiSalvatore Valitutti
Jan 27, 2010·International Reviews of Immunology·Fang Zhou
Feb 4, 1999·Cell Adhesion and Communication·S López-BrionesR González-Amaro
Sep 21, 2006·British Journal of Haematology·Jian-Qing MiThierry Bonnefoix
Mar 5, 2003·Immunological Reviews·Julie A LucasLeslie J Berg
Feb 26, 2004·The Journal of Biological Chemistry·Botond BánfiJos A Cox
Oct 28, 2016·Nature Communications·Nadia AnikeevaYuri Sykulev
Sep 17, 2009·Immunological Reviews·Arun T Pores-Fernando, Adam Zweifach
Sep 17, 2009·Immunological Reviews·Didi Matza, Richard A Flavell
Jul 2, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nico MelzerHeinz Wiendl
Jan 3, 2013·Immunological Reviews·Morgan HuseThushara P Abeyweera
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Shu HeHanne L Ostergaard
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S I GringhuisC L Verweij
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K FettucciariP Marconi
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RadojaA B Frey
Oct 31, 2002·The Journal of Biological Chemistry·Philippe GuillaumeImmanuel F Luescher
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel S JonesBrian D Evavold
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E L Tham, M F Mescher
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donald R DrakeThomas J Braciale
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elise LandaisElisabeth Houssaint
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G Z TauP B Rothman
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luana O AtherlyLeslie J Berg
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer S Y MaSasa Radoja
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoko SekiThomas J Sayers
Feb 4, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ron WolchinskyYoram Reiter

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