PMID: 7538685Jan 26, 1995Paper

Ca2+ signaling through secretagogue and growth factor receptors on pancreatic AR42J cells

Regulatory Peptides
D M SimeoneJ A Williams

Abstract

Intracellular signaling by an increase in [Ca2+]i was observed in pancreatic AR42J cells in response to agonists whose receptors are G-protein coupled including cholecystokinin (CCK), bombesin, carbachol, substance P, pituitary adenylate cyclase activating peptide (PACAP), bradykinin, ATP, calcitonin gene related peptide (CGRP), and in response to growth factors EGF and FGF whose receptors are tyrosine kinases. The response to growth factors was smaller both in magnitude and in the percentage of cells responding but was independent of extracellular Ca2+. CCK and carbachol induced sizeable increases in inositol phosphates while growth factors did not. The responses to both carbachol and EGF, however, were blocked by the phospholipase C inhibitor U73122. The tyrosine kinase inhibitor, genestein, blocked the response to EGF but not that to CCK. These data are consistent with two types of signaling mechanisms in AR42J cells. Secretagogues act on receptors which couple through G proteins to induce a large amount of inositol phosphate production and subsequent intracellular Ca2+ mobilization. Growth factors act on receptors which signal through tyrosine kinase activity and in this cell type produced limited amounts of inositol phosph...Continue Reading

References

Aug 1, 1979·Cancer Letters·D S LongneckerW Noll
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Carpenter
May 31, 1991·Biochemical and Biophysical Research Communications·B Chandrasekar, M Korc
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G BrookerC Wahlestedt
Apr 14, 1987·Biochemical and Biophysical Research Communications·C D LogsdonJ A Williams
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M I WahlG Carpenter
Jan 28, 1993·Nature·M J Berridge
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Pisegna, S A Wank

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Citations

Jan 1, 1995·Peptides·D M SimeoneM W Mulholland
Aug 12, 2003·European Journal of Pharmacology·Jack M WebsterRichard J H Wojcikiewicz
May 5, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L A DethloffL L Bestervelt
Jun 17, 1998·Molecular Biology of the Cell·B S WilsonR J Wojcikiewicz
Feb 8, 2005·The International Journal of Biochemistry & Cell Biology·Jan Kit Ching LaiPo Sing Leung
May 5, 2017·International Journal of Molecular Sciences·Joanna BoniorJolanta Jaworek
Jan 5, 2007·Journal of Cellular Biochemistry·Yannik Regimbald-DumasGaétan Guillemette
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·L ThommesenA Laegreid

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