Ca(2+) signalling by P2Y receptors in cultured rat aortic smooth muscle cells.

British Journal of Pharmacology
Sriram GovindanColin W Taylor

Abstract

P2Y receptors evoke Ca(2+) signals in vascular smooth muscle cells and regulate contraction and proliferation, but the roles of the different P2Y receptor subtypes are incompletely resolved. Quantitative PCR was used to define expression of mRNA encoding P2Y receptor subtypes in freshly isolated and cultured rat aortic smooth muscle cells (ASMC). Fluorescent indicators in combination with selective ligands were used to measure the changes in cytosolic free [Ca(2+)] in cultured ASMC evoked by each P2Y receptor subtype. The mRNA for all rat P2Y receptor subtypes are expressed at various levels in cultured ASMC. Four P2Y receptor subtypes (P2Y1, P2Y2, P2Y4 and P2Y6) evoke Ca(2+) signals that require activation of phospholipase C and comprise both release of Ca(2+) from stores and Ca(2+) entry across the plasma membrane. Combining analysis of P2Y receptor expression with functional analyses using selective agonists and antagonists, we isolated the Ca(2+) signals evoked in ASMC by activation of P2Y1, P2Y2, P2Y4 and P2Y6 receptors.

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Citations

Jul 7, 2012·Purinergic Signalling·Sriram Govindan, Colin W Taylor
Dec 14, 2011·American Journal of Physiology. Cell Physiology·Gavin E MorrisJonathon M Willets
May 10, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Takayuki MatsumotoR Clinton Webb
May 27, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Timo RiegVolker Vallon
Apr 23, 2011·American Journal of Respiratory and Critical Care Medicine·Rodolfo Paula VieiraMarco Idzko
Oct 9, 2015·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Fouzia PanhwarNoel W Davies
Jan 8, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Erika B Westcott, Steven S Segal
Sep 20, 2012·The Journal of Pharmacology and Experimental Therapeutics·Callum MitchellCharles Kennedy
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Feb 15, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Hiroshi SatonakaYasunobu Hirata
Jul 6, 2014·The Journal of Biological Chemistry·Jaroslawna MeisterAngela Schulz

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism

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