PMID: 9426303Feb 28, 1998Paper

Ca2+-transients induced by K+ channel openers in isolated coronary capillaries

Pflügers Archiv : European journal of physiology
U LangheinrichJürgen Daut

Abstract

To study the role of endothelial ATP-sensitive K+ channels in the regulation of vascular tone we examined the intracellular calcium concentration ([Ca2+]i) in coronary capillaries consisting only of endothelial cells. Coronary capillary fragments were isolated enzymatically from the guinea-pig heart and [Ca2+]i was determined by microfluorometry of fura-2 loaded cells. Low concentrations of the K+ channel opener diazoxide, which caused pronounced glibenclamide-sensitive hyperpolarization in capillaries, induced a rapid, transient rise in [Ca2+]i followed by a sustained elevation of [Ca2+]i (19 of 40 experiments). [Ca2+]i in the endothelial cells increased from 32 +/- 7 nM at rest to 66 +/- 11 nM at the peak (n = 19). One third of the [Ca2+]i-transients showed irregular oscillations of [Ca2+]i. No significant difference in the [Ca2+]i-response induced by 100 nM or 1 muM diazoxide was found. Similar results were obtained with the K+ channel opener rilmakalim. Simultaneous measurements of the membrane potential and [Ca2+]i with fluorometric methods indicated that the hyperpolarization but not the [Ca2+]i-transient could be repeatedly induced in a single capillary by the K+ channel openers. Electrophysiological recordings of the me...Continue Reading

Citations

Jun 14, 2000·European Journal of Pharmacology·R White, C R Hiley
Dec 22, 1999·British Journal of Pharmacology·T MuraiM Watanabe
Nov 14, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Prasad V G KatakamDavid W Busija
Jun 25, 2013·Pharmacology & Therapeutics·William A Coetzee
Jul 2, 2003·Journal of Pharmacological Sciences·Aleksandra NovakovićTomislav Kazić
Oct 14, 2016·Pathobiology of Aging & Age Related Diseases·Hua-Qian YangWilliam A Coetzee
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Sep 13, 2017·The Journal of Biological Chemistry·Qadeer AzizAndrew Tinker
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