Ca(2+)-triggered membrane permeability transition in deenergized mitochondria from rat liver

FEBS Letters
B V Chernyak Chernyak VYa

Abstract

The opening of the cyclosporin A-sensitive permeability transition pore (MTP) in deenergized mitochondria was induced only at millimolar Ca2+. Pretreatment of the mitochondria with 'inducers', such as duroquinone and phenylarsine oxide, allowed observing the pore opening at 0.01-0.1 mM Ca2+. Duroquinone caused a rapid (within 20 s) NAD(P)H oxidation which was followed by a slow (20 min) induction of the pore sensitive to low Ca2+. Phenylarsine oxide capable of cross-linking of vicinal SH-groups caused pore formation without the oxidation of NAD(P)H. The pore opening by both 'inducers' was prevented by N-ethylmaleimide. We propose that oxidation or cross-linking of critical dithiol(s) in membrane proteins increase the sensitivity of a putative 'Ca(2+)-sensor' that regulates the permeability transition pore opening.

References

Jul 1, 1979·Archives of Biochemistry and Biophysics·R A Haworth, D R Hunter
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May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
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Citations

Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Miriam BreunigAchim Goepferich
Oct 20, 2011·Biochimica Et Biophysica Acta·Ya-ting Tina YangSteven P Gieseg
Jul 31, 1998·Archives of Biochemistry and Biophysics·M Y Balakirev, G Zimmer
Jul 19, 2008·Toxicology Letters·N I FedotchevaN V Beloborodova

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