c.A2456C-substitution in Pck1 changes the enzyme kinetic and functional properties modifying fat distribution in pigs

Scientific Reports
Pedro LatorrePascual López-Buesa

Abstract

Cytosolic phosphoenolpyruvate carboxykinase, PCK1, is one of the main regulatory enzymes of gluconeogenesis and glyceroneogenesis. The substitution of a single amino acid (Met139Leu) in PCK1 as a consequence of a single nucleotide polymorphism (SNP), c.A2456C, is associated in the pig to a negative phenotype characterized by reduced intramuscular fat content, enhanced backfat thickness and lower meat quality. The p.139L enzyme shows reduced kcat values in the glyceroneogenic direction and enhanced ones in the anaplerotic direction. Accordingly, the expression of the p.139L isoform results in about 30% lower glucose and 9% lower lipid production in cell cultures. Moreover, the ability of this isoform to be acetylated is also compromised, what would increase its susceptibility to be degraded in vivo by the ubiquitin-proteasome system. The high frequency of the c.2456C allele in modern pig breeds implies that the benefits of including c.A2456C SNP in selection programs could be considerable.

References

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Citations

Dec 17, 2016·International Journal of Molecular Sciences·Ramona Natacha PenaJoan Estany
Nov 21, 2018·PloS One·María MuñozCristina Óvilo
Feb 9, 2020·Genetics, Selection, Evolution : GSE·Christian GroßDick de Ridder
Feb 14, 2021·International Journal of Molecular Sciences·Klavdija PoklukarMartin Škrlep

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Methods Mentioned

BETA
transgenic
acetylation
genotyping
PCR
Assay

Software Mentioned

UNICORN Manager
Imagemaster D Platinum 7
ImageJ
ClustalW
Image Scanner III
TM

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