PMID: 8986832Dec 24, 1996Paper

Ca2+/calmodulin-dependent protein kinase II phosphorylation of the presynaptic protein synapsin I is persistently increased during long-term potentiation

Proceedings of the National Academy of Sciences of the United States of America
A S NayakM D Browning

Abstract

Long-term potentiation (LTP) is an increase in synaptic responsiveness thought to be involved in mammalian learning and memory. The localization (presynaptic and/or postsynaptic) of changes underlying LTP has been difficult to resolve with current electrophysiological techniques. Using a biochemical approach, we have addressed this issue and attempted to identify specific molecular mechanisms that may underlie LTP. We utilized a novel multiple-electrode stimulator to produce LTP in a substantial portion of the synapses in a hippocampal CA1 minislice and tested the effects of such stimulation on the presynaptic protein synapsin I. LTP-inducing stimulation produced a long-lasting 6-fold increase in the phosphorylation of synapsin I at its Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) sites without affecting synapsin I levels. This effect was fully blocked by either the N-methyl-D-aspartate receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid (APV) or the CaM kinase II inhibitor KN-62. Our results indicate that LTP expression is accompanied by persistent changes in presynaptic phosphorylation, and specifically that presynaptic CaM kinase II activity and synapsin I phosphorylation may be involved in LTP expression.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Forn, P Greengard
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K D ParfittM D Browning
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R LlinásP Greengard
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D Muller, G Lynch
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D MullerG Lynch
Oct 13, 1983·Nature·E J Nestler, P Greengard
Nov 18, 1994·Science·R Malinow
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·O HvalbyP Andersen

❮ Previous
Next ❯

Citations

Jul 18, 2009·Journal of Molecular Neuroscience : MN·Eiki TakahashiChitoshi Itakura
Mar 24, 2005·Progress in Neurobiology·K A AlkadhiA M Aleisa
Feb 1, 2000·Trends in Neurosciences·T R Soderling, V A Derkach
Mar 1, 1997·Neurobiology of Aging·K E EcklesM D Browning
Jan 26, 2000·Journal of Neurochemistry·K FukunagaE Miyamoto
May 22, 2001·Journal of Neurochemistry·K T DineleyJ D Sweatt
Aug 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qingbo TangQuinn H Hogan
Dec 18, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rachel A JeffreyKelsey C Martin
Jun 10, 2014·Neurochemistry International·Lin LiGert Lubec
Aug 16, 2002·Bipolar Disorders·Maurizio PopoliGiorgio Racagni
Oct 15, 2014·The Journal of General Physiology·Matthew J Van HookWallace B Thoreson
Jan 24, 2007·The European Journal of Neuroscience·Claudie HooperSimon Lovestone
Jul 6, 2007·The European Journal of Neuroscience·Shumin LiuOttavio Arancio
Jul 1, 2008·The European Journal of Neuroscience·Solenne ChardonnetSabrina Davis
Apr 27, 2012·Journal of Neuroscience Research·Takashi KatoToshikazu Nakamura
Mar 15, 2006·Neurobiology of Disease·A M AleisaK A Alkadhi
Mar 2, 2013·PloS One·Katalin BartusJohn Garthwaite
Jun 20, 2002·Science's STKE : Signal Transduction Knowledge Environment·D R GrosshansM D Browning
Dec 12, 2001·Nature Neuroscience·D R GrosshansM D Browning
Oct 4, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Phu V TranMichael K Georgieff
Jun 20, 2018·The Journal of Pharmacology and Experimental Therapeutics·Karim A Alkadhi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.