PMID: 3760869Nov 1, 1986Paper

Ca2+/diacylglycerol-activated, phospholipid-dependent protein kinase in the Hermissenda CNS

Journal of Neurochemistry
J T NearyDaniel L Alkon

Abstract

In mammalian systems, Ca2+/diacylglycerol-activated phospholipid-dependent protein kinase (C-kinase) appears to play an important role in regulating physiological responses that outlast the transient rise in cytosolic Ca2+. Electrophysiological experiments in neurons of the nudibranch mollusc, Hermissenda crassicornis, have suggested a role for C-kinase in the long-lasting reductions in early and late K+ currents that have been observed following associative learning. Accordingly, we have investigated the catalytic properties of C-kinase in Hermissenda CNS. Following homogenization in Ca2+-free buffer, C-kinase can be separated from Ca2+/calmodulin-dependent protein kinase by centrifugation; C-kinase activity is found in the supernatant whereas essentially all of the Ca2+/calmodulin-dependent protein kinase is found in the membrane fraction. Addition of Ca2+, phosphatidylserine, and diacylglycerol to the cytosol results in phosphorylation of at least eight endogenous proteins. The Hermissenda CNS C-kinase can also phosphorylate lysine-rich histone, a substrate for mammalian C-kinase. The molluscan enzyme exhibits phospholipid specificity in that phosphatidylserine is much more effective than phosphatidylethanolamine, phosphatid...Continue Reading

References

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Citations

Mar 1, 1990·Neuroscience and Behavioral Physiology·B I Kotlyar, A S Pivovarov
Jan 1, 1988·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C C Stichel
Jan 1, 1987·Biotechnology & Genetic Engineering Reviews·S L Neidleman
Mar 13, 2014·Neurobiology of Learning and Memory·Satoshi TakigamiManabu Sakakibara
Feb 1, 1993·Journal of Neurochemistry·D L McPhieD L Alkon
Sep 1, 1988·Journal of Neurochemistry·D L AlkonH Rasmussen
Feb 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T J Nelson, D L Alkon
Jan 1, 1989·Molecular Neurobiology·B BankD L Alkon
May 25, 1991·European Journal of Pharmacology·L HegemannB H Schmidt

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