PMID: 2504060Aug 1, 1989Paper

Ca2+]i and contraction of ear artery in response to rapid stimulation by NE: measurements with quin2 and fura-2

The American Journal of Physiology
M Vonderlage, V Schreiner

Abstract

The relationship between the quin2 and fura-2 fluorescence and isovolumetric contraction of smooth muscle cells of everted segments of the rabbit ear artery in response to rapid stimulation by norepinephrine (NE) was investigated. The resting level measured with quin2 (91 +/- 10 nM, n = 4) and with fura-2 (87 +/- 9 nM, n = 5) did not significantly differ. After addition of NE, an initial slow increase (ISI) in emission could be measured with quin2; however, Ca2+ buffering by this indicator slowed the increase of intracellular Ca2+ concentration ([Ca2+]i) and of contraction. When using fura-2, no ISI could be measured, but this indicator did not significantly interfere with the normal time course of contraction. Maximal response in [Ca2+]i to 1 microM NE was 424 +/- 30 nM (with quin2) and 337 +/- 46 nM (with fura-2). Mechanical latency depended not only on the onset but also on the initial rate of the increase in [Ca2+]i. Contractile response was quickest around 0.1 s after the onset of the fura-2 signal. During its rising phase, the rate of pressure development was linearly correlated with the -log molar Ca2+ concentration (pCa) of the cytoplasm (pCai), whereas during slow relaxation, pressure was linearly related to the pCai. ...Continue Reading

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