PMID: 8945994Nov 1, 1996Paper

Ca2+]i in rat afferent arteriole during constriction measured with confocal fluorescence microscopy

The American Journal of Physiology
K P Yip, D J Marsh

Abstract

Using confocal fluorescence microscopy, we followed the time course of intracellular calcium concentration ([Ca2+]i) in vascular smooth muscle cells (VSMC) and endothelial cells (EC) of microperfused afferent arterioles isolated from rat juxtamedullary nephrons. Measurements were made while arterioles were exposed to pharmacological agonists or myogenic stimulation. Luminal addition of acetylcholine triggered an immediate increase of [Ca2+]i in EC and vasodilation. Addition of norepinephrine to the bath solution constricted the vessel and initiated periodic oscillations of [Ca2+]i in VSMC and synchronized vasomotion. Increase of perfusion pressure from 80 to 120 mmHg induced an immediate 9.6 +/- 2.9% (P < 0.05, n = 9) increase of [Ca2+]i in VSMC that was sustained. The arteriole dilated transiently when the perfusion pressure was increased, and persistent vasoconstriction to reduced diameter was observed after 35 s. When nitric oxide (NO) production in the perfused vessel was blocked with nitro-L-arginine methyl ester prior to the pressure step, similar profiles of change in VMSC [Ca2+]i were observed and persistent vasoconstriction began after 28 s. The same pressure step triggered an increase of [Ca2+]i in EC by 11.3 +/- 1.1%...Continue Reading

Citations

Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S BudelJ L Bény
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Kay-Pong YipIra Kurtz
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Max Salomonsson, William J Arendshorst
May 6, 2004·Archives of Histology and Cytology·Makoto MatsuuraYoh-ichi Satoh
Mar 21, 2008·American Journal of Physiology. Renal Physiology·N KleinstreuerZ Endre
Feb 8, 2000·American Journal of Physiology. Renal Physiology·M SalomonssonW J Arendshorst
Aug 19, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lavanya BalasubramanianKay-Pong Yip
Jul 13, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Xuemei WangWilliam A Cupples
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
Feb 15, 2001·American Journal of Physiology. Cell Physiology·W L ChanK P Yip
May 16, 2001·American Journal of Physiology. Renal Physiology·A JustH R Kirchheim
Sep 23, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Armin Just
Jun 27, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fiona HannerJános Peti-Peterdi
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A SchusterJ J Meister
Feb 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Mathieu LamboleyJean-Jacques Meister
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cheng-Han LeeCornelis van Breemen

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