PMID: 9174645May 1, 1997Paper

Ca2+]i oscillations and [Ca2+]i waves in rat megakaryocytes

Cell Calcium
S Tertyshnikova, A Fein

Abstract

ATP activated [Ca2+]i oscillations were measured in single rat megakaryocytes using fluorescence ratio microscopy. With increasing ATP concentration the duration of the [Ca2+]i oscillations increased, however, there was considerable variation from cell to cell in the absolute value of the peak [Ca2+]i and the frequency and duration of the oscillations. This variation depended, in part, on the level of Fura-2 loading suggesting that megakaryocytes are sensitive to buffering of [Ca2+]i by Fura-2. Agents, that increase the level of intracellular cGMP (sodium nitroprusside and 8-pCPT-cGMP) or cAMP (prostacyclin, IBMX, forskolin and 8-bromo-cAMP) inhibited [Ca2+]i oscillations. Despite the large cell to cell variation in the patterns of [Ca2+]i oscillations, reapplication of the agents that elevated cAMP or cGMP inhibited the oscillations similarly. Using video rate fluorescence ratio imaging we found that the agonist-induced [Ca2+]i oscillations were the result of a well-defined [Ca2+]i wave, which spread across the cell with an average speed of about 35 microns/s, during the rising phase of each oscillatory spike. After reaching a peak, [Ca2+]i decreased uniformly across the whole cell during the falling phase of the spike. Analys...Continue Reading

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J GeigerU Walter
Jul 15, 1992·European Journal of Biochemistry·B Brüne, V Ullrich
Mar 16, 1992·Biochemical and Biophysical Research Communications·Y OzakiS Kume
Feb 1, 1991·Cell Calcium·K S Cuthbertson, T R Chay
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·T Meyer, L Stryer
Feb 1, 1991·Cell Calcium·S Miyazaki
Jan 1, 1990·Annual Review of Physiology·T J Rink, S O Sage
May 22, 1990·Biochimica Et Biophysica Acta·R Jacob
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A GoldbeterM J Berridge
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T Meyer, L Stryer
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Jan 1, 1989·Reviews of Physiology, Biochemistry and Pharmacology·U Walter
Jul 26, 1988·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M J BerridgeK S Cuthbertson
Jul 26, 1988·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R PayneA Fein
Feb 1, 1974·The Journal of General Physiology·J M Russell, M P Blaustein
May 10, 1974·Nature·R D Feinman, T C Detwiler
Nov 1, 1982·Biophysical Journal·R B SteinJ Shriver
Oct 15, 1994·The Journal of Physiology·B Somasundaram, M P Mahaut-Smith
Jul 14, 1995·The Journal of Biological Chemistry·B Somasundaram, M P Mahaut-Smith
Jan 1, 1994·Pharmacology & Therapeutics·P Sargeant, S O Sage
Nov 7, 1994·Neuroscience Letters·P Zerr, A Feltz

❮ Previous
Next ❯

Citations

Oct 16, 2002·Cell Calcium·L Jaffe
Mar 29, 2000·The Journal of General Physiology·S Tertyshnikova, A Fein
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Tertyshnikova, A Fein
Oct 13, 2006·Biophysical Chemistry·C SardetA McDougall
Oct 5, 2010·Cell Calcium·Lionel F Jaffe
Dec 4, 2001·The Journal of Physiology·D ThomasM P Mahaut-Smith
Jul 17, 1999·The American Journal of Physiology·J H Evans, M J Sanderson
Jan 23, 2020·Science Signaling·Alexander P ByeMartyn P Mahaut-Smith
Nov 13, 2001·American Journal of Physiology. Renal Physiology·H Y KwanX Yao
Oct 30, 1998·Cell Calcium·L F Jaffe, R Créton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.