PMID: 7543476Aug 1, 1995Paper

CadC, the transcriptional regulatory protein of the cadmium resistance system of Staphylococcus aureus plasmid pI258

Journal of Bacteriology
G Endo, S Silver

Abstract

The CadC protein from the cadA cadmium resistance operon of Staphylococcus aureus plasmid pI258 regulates transcription of this system in vitro. The CadC protein was overproduced in Escherichia coli cells and partially purified. Gel shift assays of the proposed cadA operator/promoter region DNA showed specific association with the CadC protein. Control arsenic resistance operator/promoter DNA from the same plasmid was not shifted by the CadC protein. Cd2+, Bi3+, and Pb2+ caused the release of CadC from DNA in gel retardation assays. DNase I footprinting measurements showed that the CadC protein specifically associated with and protected a region of operator/promoter DNA from nucleotide positions -7 to +14 relative to the start point of mRNA synthesis. Runoff transcription assays with the operator/promoter region of DNA (plus the first 69 nucleotides of the cadC gene) and purified E. coli RNA polymerase gave an mRNA product of the predicted size. Added CadC protein inhibited transcription in vitro.

References

May 1, 1992·Journal of Bacteriology·A O Summers
Mar 1, 1992·Microbiological Reviews·S Silver, M Walderhaug
Feb 1, 1989·Trends in Biochemical Sciences·S SilverT K Misra
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G NuciforaS Silver
Apr 1, 1968·Journal of Bacteriology·R P Novick, C Roth
Aug 6, 1993·Science·T V O'Halloran
Sep 1, 1993·Archives of Biochemistry and Biophysics·K J Tsai, A L Linet

❮ Previous
Next ❯

Citations

Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
May 8, 2003·Analytical and Bioanalytical Chemistry·Ranjit S ShettySylvia Daunert
Jan 25, 2006·Molecular Microbiology·Duncan R HarvieNigel J Robinson
Jun 30, 2007·The Journal of Biological Chemistry·Lucia BanciNigel J Robinson
Jan 1, 1996·Annual Review of Microbiology·S Silver, L T Phung
Feb 27, 1999·Molecular Microbiology·K R BrocklehurstA P Morby
Apr 4, 1997·The Journal of Biological Chemistry·D Strausak, M Solioz
May 6, 2010·Natural Product Reports·Deenah Osman, Jennifer S Cavet
Oct 8, 2008·The Biochemical Journal·Francesca CantiniMarc Solioz
Jun 10, 2016·FEMS Microbiology Letters·Doriana Mădălina VoicaAharon Oren
May 18, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rudra P SahaManoj Kumar Singh
Mar 17, 2009·The Journal of Biological Chemistry·Ashoka KandegedaraBarry P Rosen
Dec 19, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Ran Friedman
Mar 28, 2013·Archaea : an International Microbiological Journal·Pallavee Srivastava, Meenal Kowshik
Apr 15, 2014·Archaea : an International Microbiological Journal·Joseph R PetersonZaida Luthey-Schulten
Jan 9, 2018·Nucleic Acids Research·Carlos J CaballeroAlejandro Toledo-Arana
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·C ThelwellJ S Turner-Cavet
Apr 6, 2002·FEMS Microbiology Letters·I M Solovieva, K D Entian
Feb 14, 2006·FEMS Microbiology Reviews·Susan MillsR Paul Ross
Sep 20, 2012·The Journal of General and Applied Microbiology·Pongsak Rattanachaikunsopon, Parichat Phumkhachorn
Nov 26, 1998·The Journal of Biological Chemistry·C RensingB P Rosen
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mario A PennellaDavid P Giedroc
Jun 29, 1999·Journal of Bacteriology·S S CrupperJ J Iandolo
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Aug 9, 2005·Applied and Environmental Microbiology·Naghma NazGeoffrey M Gadd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.