Cadherin-23, myosin VIIa and harmonin, encoded by Usher syndrome type I genes, form a ternary complex and interact with membrane phospholipids.

Human Molecular Genetics
Amel BahloulChristine Petit

Abstract

Cadherin-23 is a component of early transient lateral links of the auditory sensory cells' hair bundle, the mechanoreceptive structure to sound. This protein also makes up the upper part of the tip links that control gating of the mechanoelectrical transduction channels. We addressed the issue of the molecular complex that anchors these links to the hair bundle F-actin core. By using surface plasmon resonance assays, we show that the cytoplasmic regions of the two cadherin-23 isoforms that do or do not contain the exon68-encoded peptide directly interact with harmonin, a submembrane PDZ (post-synaptic density, disc large, zonula occludens) domain-containing protein, with unusually high affinity. This interaction involves the harmonin Nter-PDZ1 supramodule, but not the C-terminal PDZ-binding motif of cadherin-23. We establish that cadherin-23 directly binds to the tail of myosin VIIa. Moreover, cadherin-23, harmonin and myosin VIIa can form a ternary complex, which suggests that myosin VIIa applies tension forces on hair bundle links. We also show that the cadherin-23 cytoplasmic region, harmonin and myosin VIIa interact with phospholipids on synthetic liposomes. Harmonin and the cytoplasmic region of cadherin-23, both independe...Continue Reading

References

Dec 1, 1988·The American Journal of Physiology·D M ShasbyS S Shasby
Jun 9, 2001·American Journal of Human Genetics·Z M AhmedE R Wilcox
Aug 7, 2001·Human Molecular Genetics·K N AlagramamG S Hagemen
Oct 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jan SiemensUlrich Müller
Dec 6, 2002·The Journal of Biological Chemistry·Akira Inoue, Mitsuo Ikebe
Dec 3, 2003·Vision Research·Daniel Bindman, Charles Chubb
Dec 14, 2004·Human Molecular Genetics·Avital AdatoChristine Petit
Mar 19, 2005·The Journal of Comparative Neurology·Richard J GoodyearGuy P Richardson
Sep 28, 2005·The Journal of Biological Chemistry·Arnon Henn, Enrique M De La Cruz
Feb 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mathias SenftenUlrich Müller
Jun 30, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zubair M AhmedThomas B Friedman
Jul 11, 2007·Biochemistry·Per Jemth, Stefano Gianni
Feb 3, 2009·Current Topics in Developmental Biology·Michel LeiboviciChristine Petit
Mar 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lifeng PanMingjie Zhang
Mar 31, 2009·Nature Neuroscience·Maryline BeurgAnthony J Ricci
May 19, 2009·Neuron·Nicolas GrilletUlrich Müller
May 28, 2009·Nature Neuroscience·Christine Petit, Guy P Richardson
Aug 12, 2009·Hepatology International·Hoda A MakhloufHebat Alla G Rashed
Sep 17, 2009·Pflügers Archiv : European journal of physiology·Nicolas MichalskiChristine Petit
Oct 7, 2009·Cell·Peter G Gillespie, Ulrich Müller
Jan 22, 2010·Chembiochem : a European Journal of Chemical Biology·Rodrigo GallardoPascale Zimmermann
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jing YanMingjie Zhang

❮ Previous
Next ❯

Citations

Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhiyi WeiMingjie Zhang
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Elisa CaberlottoChristine Petit
Mar 3, 2012·Human Molecular Genetics·Qing Yin ZhengPeter G Gillespie
May 17, 2011·Orphanet Journal of Rare Diseases·Crystel BonnetFrançoise Denoyelle
Jul 16, 2011·Disease Models & Mechanisms·Jennifer B PhillipsMonte Westerfield
Oct 11, 2012·PloS One·Hongyu ZhaoPeter G Gillespie
Apr 8, 2014·Comptes rendus biologies·Aziz El-Amraoui, Christine Petit
Nov 16, 2010·Annual Review of Physiology·Guy P RichardsonChristine Petit
Mar 15, 2014·Disease Models & Mechanisms·Bernardo Blanco-SánchezMonte Westerfield
Oct 10, 2012·The Journal of Cell Biology·Iman SahlyChristine Petit
Jul 17, 2012·Trends in Neurosciences·Guy P Richardson
Dec 20, 2011·Trends in Neurosciences·Piotr Kazmierczak, Ulrich Müller
Jun 7, 2015·Hearing Research·Elise Pepermans, Christine Petit
Dec 24, 2010·Annals of the New York Academy of Sciences·Dorith RavivKaren B Avraham
Nov 19, 2013·The International Journal of Biochemistry & Cell Biology·Dominic Cosgrove, Marisa Zallocchi
Sep 8, 2012·Bioarchitecture·Katy C Liu, Richard E Cheney
Dec 8, 2014·Biochimica Et Biophysica Acta·Pranav Mathur, Jun Yang
Jun 25, 2014·Pflügers Archiv : European journal of physiology·Nicolas Michalski, Christine Petit
Sep 23, 2014·Pflügers Archiv : European journal of physiology·Thomas EffertzAnthony J Ricci
Nov 12, 2016·Current Opinion in Cell Biology·Meredith L WeckMatthew J Tyska
Jul 1, 2017·Nature Communications·I-Mei YuAnne Houdusse
Aug 30, 2017·Cold Spring Harbor Perspectives in Biology·Avinash JaiganeshMarcos Sotomayor
Nov 8, 2018·Molecular Biology of the Cell·Julia A PinetteMatthew J Tyska
Feb 9, 2012·Physiology·Lifeng Pan, Mingjie Zhang
Nov 1, 2017·EMBO Molecular Medicine·Vincent MichelAziz El-Amraoui
Jul 6, 2014·Physiological Reviews·Robert Fettiplace, Kyunghee X Kim
Jan 9, 2019·Cold Spring Harbor Perspectives in Medicine·Guy P Richardson, Christine Petit
Dec 29, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Qi YaoXue Zhong Liu
May 13, 2017·The Journal of Cell Biology·Cataldo SchietromaChristine Petit
Sep 7, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rayna RosatiManohar Ratnam
Nov 29, 2020·Developmental Biology·Jennifer L SalleeDaniel P Kiehart
Nov 17, 2020·Frontiers in Genetics·Meg WhatleyElaine Y M Wong

❮ Previous
Next ❯

Methods Mentioned

BETA
pull-down
surface plasmon resonance
circular dichroism
NMR
total correlated spectroscopy
biosensor
size-exclusion chromatography
transfection
dissection

Software Mentioned

Igor Pro

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.