Cadherin is involved in the action of Bacillus thuringiensis toxins Cry1Ac and Cry2Aa in the beet armyworm, Spodoptera exigua

Journal of Invertebrate Pathology
Lin QiuChaoliang Lei

Abstract

Bacillus thuringiensis (Bt) insecticidal crystal (Cry) proteins are effective against some insect pests in sprays and transgenic crops, although the evolution of resistance could threaten the long-term efficacy of such Bt use. One strategy to delay resistance to Bt crops is to "pyramid" two or more Bt proteins that bind to distinct receptor proteins within the insect midgut. The most common Bt pyramid in cotton (Gossypium hirsutum L.) employs Cry1Ac with Cry2Ab to target several key lepidopteran pests, including the beet armyworm, Spodoptera exigua (Hübner), which is a serious migratory pest of many vegetable crops and is increasingly important in cotton in China. While cadherin and aminopeptidase-N are key receptors of Cry1 toxins in many lepidopterans including S. exigua, the receptor for Cry2A toxins remains poorly characterized. Here, we show that a heterologous expressed peptide corresponding to cadherin repeat 7 to the membrane proximal extracellular domain (CR7-MPED) in the S. exigua cadherin 1b (SeCad1b) binds Cry1Ac and Cry2Aa. Moreover, SeCad1b transcription was suppressed in S. exigua larvae by oral RNA interference and susceptibility to Cry1Ac and Cry2Aa was significantly reduced. These results indicate that SeCad1b...Continue Reading

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Citations

Mar 5, 2016·Frontiers in Plant Science·Ibrokhim Y AbdurakhmonovAlan E Pepper
Jul 21, 2016·Current Opinion in Insect Science·Salvador HerreroJuan Ferré
Nov 13, 2015·Scientific Reports·Jeffrey A FabrickBruce E Tabashnik
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May 10, 2019·International Journal of Molecular Sciences·Muhammad HafeezMo Wang
Jul 2, 2019·Archives of Insect Biochemistry and Physiology·Gabriela da SilvaDanielle Maria Perpétua de Oliveira
Feb 2, 2019·Applied Biochemistry and Biotechnology·Camila Soares FigueiredoJanete Apparecida Desidério
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Feb 6, 2020·Archives of Insect Biochemistry and Physiology·Bingjie WangGemei Liang
Oct 29, 2021·Environmental Science and Pollution Research International·Muhammad HafeezYaobin Lu

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