Cadmium-induced crystallization of proteins: II. Crystallization of the Salmonella typhimurium histidine-binding protein in complex with L-histidine, L-arginine, or L-lysine

Protein Science : a Publication of the Protein Society
S TrakhanovB Rupp

Abstract

To further investigate favorable effects of divalent cations on the formation of protein crystals, three complexes of Salmonella typhimurium histidine-binding protein were crystallized with varying concentrations of cadmium salts. For each of the three histidine-binding protein complexes, cadmium cations were found to promote or improve crystallization. The optimal cadmium concentration is ligand specific and falls within a narrow concentration range. In each case, crystals grown in the presence of cadmium diffract to better than 2.0 angstroms resolution and belong to the orthorhombic space group P2(1)2(1)2(1). From our results and from the analysis of cadmium sites in well-refined protein structures, we propose that cadmium addition provides a generally useful technique to modify crystal morphology and to improve diffraction quality.

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Citations

Sep 6, 2012·Acta Crystallographica. Section D, Biological Crystallography·Quentin FlorenceJohn P Rose
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Guido HansenRolf Hilgenfeld
May 2, 2013·Acta Crystallographica. Section D, Biological Crystallography·Xiaolu Linda ZhangMark Paetzel
Apr 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Cameron MuraDavid Eisenberg
Jun 24, 2014·Crystal Growth & Design·C M GillespieA M Lenhoff
Jun 24, 2017·Protein Science : a Publication of the Protein Society·Raghurama P HegdeUdupi A Ramagopal
Aug 2, 2014·Acta Crystallographica. Section D, Biological Crystallography·Simon E KolstoeStephen P Wood
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Jun 10, 2005·Glycobiology·Jennifer L MeagherJeanne A Stuckey
Jul 12, 2017·Acta Crystallographica. Section D, Structural Biology·Saravanan PanneerselvamAlke Meents
Dec 19, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Ran Friedman
Jun 10, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Tsutomu NakamuraKoichi Uegaki
Nov 20, 2018·F1000Research·Igor E EliseevOleg B Chakchir
Apr 16, 2019·F1000Research·Igor E EliseevOleg B Chakchir
Aug 27, 2019·International Journal of Biological Macromolecules·Mahima Sharma, Vinay Kumar

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