Cadmium modulates biofilm formation by Staphylococcus epidermidis

International Journal of Environmental Research and Public Health
Xueqing WuJohanna Fink-Gremmels

Abstract

The aim of the study was to evaluate the effect of cadmium exposure on Staphylococcus epidermidis (ATCC 35984) biofilm formation. Bacteria were cultured in the absence or presence of different concentrations (0-50 µM) of cadmium. Biofilm formation and bacterial viability were assessed. Quantitative Real Time-PCR (qRT-PCR) was used to determine the mRNA expression of molecular markers of S. epidermidis biofilm formation and dispersion. S. epidermidis biofilm formation was stimulated (p<0.001) by 1.56 and 3.13 µM cadmium. Confocal laser scanning microscopy (CLSM) analysis confirmed an increase in biofilm thickness (23 and 22 µm, versus 17.8 µm in the controls) after exposure to 1.56 or 3.13 µM cadmium, respectively. qRT-PCR was performed showing the up-regulation of atlE, embp, aap, icaA and icaB after exposure to 3.13 µM cadmium. Taken together, these findings show that cadmium at low, sub-toxic concentrations acts as inducer of S. epidermidis biofilm formation.

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Citations

Jan 12, 2021·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Mudsser AzamQazi Mohd Rizwanul Haq

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Methods Mentioned

BETA
confocal scanning laser
environmental stress

Software Mentioned

Image J
Graph Pad Prism
Graph Pad
MyiQ System
image
MyiQ

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