Cadmium perturbs calcium homeostasis in rat osteosarcoma (ROS 17/2.8) cells; a possible role for protein kinase C

Toxicology Letters
G J Long

Abstract

The mechanism of the toxic effects of Cd2+ on bone cell function is not completely understood at this time. This study was designed to characterize the effect of Cd2+ on Ca2+ metabolism in ROS 17/2.8 cells. Cells were labeled with (45)Ca (1.87 mM Ca) for 20 h in the presence of 0.01, 0.1, or 1.0 microM Cd2+ and kinetic parameters were determined from (45)Ca efflux curves. Three kinetic compartments described the intracellular metabolism of (45)Ca. Cd2+ (0.01 microM) caused an approximate 9 x increase in Ca2+ flux across the plasma membrane and a decrease in the most rapidly exchanging intracellular Ca2+ compartment (S1). However, there was no change in total cell Ca2+, indicating an increased cycling of Ca2+ across the plasma membrane. Flux between S1 and the intermediate Ca2+ compartment (S2) was also increased and S2 increased significantly. All Cd2+ induced changes in Ca2+ homeostasis were obliterated by concurrent treatment with 0.1 microM calphostin C (CC), a potent protein kinase C (PKC) inhibitor. This data suggests that Cd2+ perturbs Ca2+ metabolism via a PKC dependent process.

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Citations

Mar 17, 2007·Journal of Toxicology and Environmental Health. Part a·K G CoonseS J Heggland
Aug 7, 2012·Journal of Cell Communication and Signaling·Jagna Chmielowska-Bąk, Joanna Deckert
Nov 20, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Spenser S SmithSara J Heggland
Sep 12, 2020·PloS One·Taichi MutohMakoto Kakinaka
Mar 13, 2014·Analytical Chemistry·Sabrina HengAndrew D Abell

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