PMID: 2500975Jul 11, 1989Paper

Cadmium stimulates prostaglandin E2 synthesis in osteoblast-like cells, MC3T3-E1

Biochimica Et Biophysica Acta
Y SuzukiS Murota

Abstract

The effect of Cd on prostaglandin E2 production in osteoblasts was studied using cloned osteoblast-like cells, MC3T3-E1, which were established from new-born mouse calvaria. Treatment of the cells with Cd caused a dose- (0-10 microM) and time- (0-24 h) dependent increase in the release of prostaglandin E2 from the cells into the culture medium. A lag time of 4 h was required for the onset of the phenomenon. The release of [14C]arachidonic acid from prelabeled cell membrane was little influenced by the Cd treatment, while conversion of [14C]arachidonic acid to prostaglandin E2 by the homogenate of the cells treated with Cd was enhanced as compared to that by untreated cells. The stimulatory effect of Cd on prostaglandin E2 production was abolished in the presence of cycloheximide (100 ng/ml). By Western blot analysis with polyclonal rabbit anti-cyclooxygenase antibody, it was revealed that Cd treatment augmented the amount of immunoreactive cyclooxygenase in the cells. These results strongly suggest that Cd stimulates prostaglandin E2 production through the induction of cyclooxygenase in MC3T3-E1 cells. This effect of Cd may be involved in the mechanisms of Cd-induced bone injury.

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Citations

Feb 1, 1994·Calcified Tissue International·T MiyaharaK Yokoyama
Aug 1, 1991·Bulletin of Environmental Contamination and Toxicology·T MiyaharaS Kuze
Oct 28, 2008·Biological Trace Element Research·Kaouthar KessabiImed Messaoudi
May 30, 2009·Environmental Health Perspectives·Manish AroraRobert O Wright
Oct 1, 1992·Toxicology and Applied Pharmacology·A CárdenasR Lauwerys
Apr 1, 1992·Toxicology and Applied Pharmacology·N Sacco-GibsonM Bhattacharyya
Oct 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·W SakamotoS Murota
Jan 17, 2015·Environmental Research·D G SfakianakisA M Tsatsakis
Jun 1, 1997·Clinical Orthopaedics and Related Research·J Y WangD T Tsukayama
Jan 24, 2021·The Science of the Total Environment·Yonggang MaZongping Liu

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