Cadmium up-regulates transcription of the steroidogenic acute regulatory protein (StAR) gene through phosphorylated CREB rather than SF-1 in K28 cells

The Journal of Toxicological Sciences
Soo-Yun ParkJ Soh

Abstract

Cadmium is a widely used heavy metal in industry and affects the male reproductive system of animals, including humans, as a result of occupational and environmental exposures. However, the molecular mechanism underlying its effect on steroidogenesis in gonads remains unclear. In this study, we demonstrated that exposure of K28 mouse testicular Leydig tumor cells to cadmium led to a significant increase in the mRNA level, promoter activity and protein level of the steroidogenic acute regulatory protein (StAR), an essential factor for steroid biosynthesis. It has been well documented that StAR gene transcription is regulated by multiple transcription factors, including cAMP-responsive element binding protein (CREB) family members and SF-1. Cadmium treatment caused an increase in CREB phosphorylation but did not alter the CREB protein level in the nucleus. EMSA studies revealed that cadmium-induced phosphorylated CREB formed specific complexes with the proximal region of the StAR gene promoter. Furthermore, co-transfection with a CREB expression plasmid significantly increased cadmium-induced StAR promoter activity. However, the nuclear level and the affinity of SF-1 protein for the StAR proximal promoter were dramatically decrea...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M R MontminyR H Goodman
Jul 1, 1985·Journal of Inorganic Biochemistry·D B CookS S Ershadi
Jan 1, 1983·Methods in Enzymology·J D DignamR G Roeder
Aug 1, 1995·Kidney International·M Matsuoka, K M Call
Nov 1, 1994·Reproductive Toxicology·M Piasek, J W Laskey
May 1, 1993·Reproductive Toxicology·B VargaG Ungváry
Oct 27, 1997·Journal of Applied Toxicology : JAT·K PaksyG S Kaáli
Apr 30, 1999·Molecular and Cellular Endocrinology·H K LeeJ Soh
Jun 11, 1999·Journal of Applied Toxicology : JAT·M Piasek, J W Laskey
Jul 20, 1999·Molecular and Cellular Endocrinology·A J ReinhartD M Stocco
May 3, 2002·Toxicology·Myeong Jin LeeKimiaki Sumino
Oct 25, 2002·International Archives of Occupational and Environmental Health·Martina PiasekMaja Blanusa
Dec 4, 2003·Mutation Research·Michael P Waalkes
Apr 21, 2004·Experimental Biology and Medicine·Michael C Henson, P Jorge Chedrese
Jun 30, 2004·Molecular and Cellular Endocrinology·Ronojoy Sen GuptaJaemog Soh
Feb 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert A GoyerMichael P Waalkes
Mar 28, 2008·Reproductive Toxicology·Jennifer Thompson, John Bannigan
Apr 24, 2012·Biochemical and Biophysical Research Communications·Mio KondoMasato Matsuoka

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