Cadmium uptake in rat hepatocytes in relation to speciation and to complexation with metallothionein and albumin

Journal of Cellular Physiology
T N D PhamC Jumarie

Abstract

Cadmium (Cd) uptake has been studied in primary cultures of rat hepatocytes focusing on the impact of inorganic and organic speciation. Uptake time-course studies over a 60-min exposure to 0.3 microM (109)Cd revealed a zero-time uptake and a slower process of accumulation which proceeds within minutes. (109)Cd uptake showed saturation kinetics (K(m) = 3.5 +/- 0.8 microM), and was highly sensitive to inhibition by Zn and Hg. There was no evidence for sensitivity to the external pH nor for any preferential transport of the free cation Cd(2+) over CdCl(n) (2-n) chloro-complexes. According to the assumption that only inorganic metal species are available, metal uptake decreased upon albumin (BSA) addition to the exposure media. In contrast, higher levels of (109)Cd accumulation were obtained under optimal conditions for Cd complexation by MT. Comparison among uptake data obtained under inorganic and organic conditions revealed that Cd-MT would be taken up 0.4 times as rapidly as Cd(inorg). We conclude that uptake of Cd in rat hepatocytes involves specific transport mechanism(s) subjected to Zn or Hg interactions. Uptake of inorganic Cd is not proportional to the levels of free Cd(2+) and does not involve the divalent cation transpo...Continue Reading

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Citations

Jun 16, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ivan SabolićCarol M Herak-Kramberger
Mar 23, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pascale CarrièreCatherine Jumarie
Apr 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·George FotakisJohn A Timbrell
Sep 15, 2018·Journal of Applied Toxicology : JAT·Magdalena Mężyńska, Malgorzata M Brzóska
Nov 18, 2006·Journal of Cellular Physiology·Qiang HaoWolfgang Maret
Oct 23, 2018·Social Work in Health Care·Rebecca S Rouland PolmanteerCarol Brownstein-Evans
Dec 21, 2019·Journal of Biochemical and Molecular Toxicology·Joannie LemaireCatherine Jumarie

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