Cadmium, vectorial active transport, and MT-3-dependent regulation of cadherin expression in human proximal tubular cells

Toxicological Sciences : an Official Journal of the Society of Toxicology
Chandra S BathulaSeema Somji

Abstract

Previous studies from this laboratory have implicated the expression of the third isoform of metallothionein (MT-3) in the maintenance of proximal tubular vectorial active ion transport. It was shown that HK-2 cells have no expression of MT-3 and do not form domes in culture; whereas, the human proximal tubular (HPT) cells and HK-2 cells stably transfected with MT-3 [HK-2(MT-3)] form these structures. In the present study, this association was further explored by determining the effect of MT-3 expression on the expression of the E -, P -, N -, K -, and Ksp-cadherins. It was demonstrated that the HPT cells and HK-2(MT-3) cells had significant elevations in the expression of messenger RNA and protein for the E -, P -, and Ksp-cadherins compared with that of the HK-2 cells transfected with the blank vector [HK-2(blank vector)]. In contrast, the HK-2(blank vector) cells had significantly elevated expression of N- and K-cadherin compared with both the HPT and HK-2(MT-3) cell lines. These patterns of cadherin expression provide strong evidence that MT-3 might be involved in epithelial to mesenchymal transition that is postulated to occur during several disease states and in the mesenchymal to epithelial transition that occurs during ...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R D PalmiterD M Durnam
Dec 31, 1991·Biochemical and Biophysical Research Communications·W C Prozialeck, R J Niewenhuis
Jan 1, 1991·Toxicology and Applied Pharmacology·W C Prozialeck, R J Niewenhuis
Oct 1, 1988·Molecular and Cellular Biochemistry·B R StevensonS Bullivant
Feb 1, 1984·Kidney International·C J DetrisacD A Sens
Sep 30, 1993·Biochemical and Biophysical Research Communications·J C WuR M DePhilip
Jan 1, 1997·Developmental Biology·G L RadiceR O Hynes
Jun 4, 1999·Toxicology Letters·S H GarrettD A Sens
May 9, 2000·Toxicology and Applied Pharmacology·W C Prozialeck
Sep 7, 2001·Current Opinion in Cell Biology·A Nagafuchi
Feb 13, 2002·Molecular and Cellular Biology·Ulf DahlHenrik Semb
Feb 19, 2002·Kidney International·Doyeob KimDonald A Sens
Aug 17, 2002·Toxicology and Applied Pharmacology·Walter C ProzialeckAlan R Parrish
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
May 7, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Seema SomjiDonald A Sens
Sep 7, 2004·The International Journal of Developmental Biology·Héctor PeinadoAmparo Cano
Mar 3, 2005·Protein Science : a Publication of the Protein Society·Geoffrey K HomStephen L Mayo
Apr 2, 2005·Protein Science : a Publication of the Protein Society·David W LahtiIan M Armitage
Apr 12, 2005·Advanced Drug Delivery Reviews·Jun Miyoshi, Yoshimi Takai
Dec 13, 2005·American Journal of Physiology. Renal Physiology·David B N LeeHarry J Ward

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Citations

Dec 2, 2010·Journal of Toxicology and Environmental Health. Part a·Scott H GarrettDonald A Sens
Mar 1, 2012·World Journal of Gastroenterology : WJG·Ferenc Sipos, Orsolya Galamb
Nov 21, 2014·International Journal of Molecular Sciences·Lawrence H LashBavneet Benipal
Jun 29, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Emmy Van KerkhoveQuirine Swennen
Sep 28, 2012·Pflügers Archiv : European journal of physiology·Sarah E JenkinsonColin D A Brown

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