CADp44: a novel regulatory subunit of the 26S proteasome and the mammalian homolog of yeast Sug2p

Gene
V W BauerM T Andrews

Abstract

We have identified a novel protein, CADp44, based on the analysis of cDNAs derived from the brainstem of the 13-lined ground squirrel, Spermophilus tridecemlineatus. CADp44 has an unmodified molecular mass of 44,178 Da and contains multiple functional domains, including a conserved ATPase domain (CAD) and a leucine zipper motif. We show that distinct regions of the CADp44 sequence are identical to a set of peptides prepared from a recently identified bovine protein, referred to as p42, which is found in the PA700 regulatory complex of the 26S proteasome (DeMartino et al., 1996). We also show that CADp44 is the functional homolog of the newly characterized Sug2 protein from the budding yeast, Saccharomyces cerevisiae (Russell et al., 1996). Consistent with its role as a component of the 26S proteasome, CADp44 mRNA is found in all ground squirrel tissues examined. Evolutionary relationships based on sequence analysis show that both CADp44 and yeast Sug2p are distinct from the other five CAD ATPases found in the PA700, and together comprise the sixth and newest CAD subunit of the regulatory complex of the 26S proteasome.

References

Jun 1, 1991·Current Opinion in Cell Biology·H Meisner, M P Czech
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Confalonieri, M Duguet
Oct 7, 1994·Cell·A Ciechanover
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B OhanaC A Rosen
Feb 9, 1996·The Journal of Biological Chemistry·G N DeMartinoC A Slaughter
Dec 20, 1996·The Journal of Biological Chemistry·S J RussellS A Johnston

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Citations

Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·B W ParkM I Greene
Jan 27, 1998·The Journal of Biological Chemistry·C SearsT Maniatis
Dec 20, 1996·The Journal of Biological Chemistry·S J RussellS A Johnston
Jun 20, 1998·Molecular and Cellular Biology·M H GlickmanD Finley

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