Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediates.

PLoS Genetics
Takamune T SaitoMónica P Colaiácovo

Abstract

Homologous recombination (HR) is essential for the repair of blocked or collapsed replication forks and for the production of crossovers between homologs that promote accurate meiotic chromosome segregation. Here, we identify HIM-18, an ortholog of MUS312/Slx4, as a critical player required in vivo for processing late HR intermediates in Caenorhabditis elegans. DNA damage sensitivity and an accumulation of HR intermediates (RAD-51 foci) during premeiotic entry suggest that HIM-18 is required for HR-mediated repair at stalled replication forks. A reduction in crossover recombination frequencies-accompanied by an increase in HR intermediates during meiosis, germ cell apoptosis, unstable bivalent attachments, and subsequent chromosome nondisjunction-support a role for HIM-18 in converting HR intermediates into crossover products. Such a role is suggested by physical interaction of HIM-18 with the nucleases SLX-1 and XPF-1 and by the synthetic lethality of him-18 with him-6, the C. elegans BLM homolog. We propose that HIM-18 facilitates processing of HR intermediates resulting from replication fork collapse and programmed meiotic DSBs in the C. elegans germline.

References

Sep 1, 1979·Molecular & General Genetics : MGG·H R HorvitzR K Herman
Jul 1, 1979·The Journal of Cell Biology·M Chalfie, J N Thomson
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B S BakerP D Smith
May 24, 1991·Science·A LupasJ Stock
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R S ChagantiJ German
May 1, 1974·Genetics·S Brenner
Dec 5, 1974·Journal of Molecular Biology·H F EpsteinS Brenner
Oct 20, 1995·The Journal of Biological Chemistry·S KleffR Sternglanz
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·M VidalJ D Boeke
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·M VidalE Harlow
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·N SugawaraJ E Haber
Dec 18, 1998·The Journal of Biological Chemistry·M C Whitby, J Dixon
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·F Pâques, J E Haber
Apr 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ItoY Sakaki
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·D M Lilley, M F White
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·A J Walhout, M Vidal
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S L GasiorD K Bishop
Dec 14, 2001·Molecular Cell·X B ChenC H McGowan
Jan 5, 2002·Science·Simon J BoultonMarc Vidal
Sep 14, 2002·Current Genetics·Vivek Kaliraman, Steven J Brill
May 16, 2003·The Journal of Biological Chemistry·Jong Sook Ahn, Matthew C Whitby
Jul 2, 2003·Genes & Development·William M Fricke, Steven J Brill
Oct 7, 2003·Molecular Biology of the Cell·Stéphane CoulonPaul Russell
Dec 4, 2003·Mutation Research·Yves PommierKurt W Kohn

❮ Previous
Next ❯

Citations

Apr 29, 2014·Methods : a Companion to Methods in Enzymology·Zebulin Kessler, Judith Yanowitz
Oct 29, 2010·Journal of Nucleic Acids·Rihito MoritaSeiki Kuramitsu
Mar 6, 2010·Genes & Development·Jennifer M Svendsen, J Wade Harper
Nov 6, 2010·Chromosoma·Bennie B L G Lemmens, Marcel Tijsterman
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kimiyo N YamamotoKouji Hirota
Apr 23, 2013·Environmental Health Perspectives·Patrick AllardMonica P Colaiácovo
May 25, 2010·FEBS Letters·Carrie A Adelman, Simon J Boulton
Apr 7, 2012·Experimental Cell Research·Adriana La Volpe, Marco Barchi
Nov 18, 2011·Molecular Biology International·Hannes Lans, Wim Vermeulen
Nov 23, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mamiko YajimaGary M Wessel
Sep 3, 2013·Cell Reports·Jamie S J WilsonJohn Rouse
Jul 25, 2014·Worm·Takamune T Saito, Monica P Colaiácovo
Mar 23, 2017·Nature Reviews. Molecular Cell Biology·Pierre-Marie Dehé, Pierre-Henri L Gaillard
Oct 4, 2017·Molecular & Cellular Proteomics : MCP·Emigdio D ReyesMatthew D Weitzman
Dec 10, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Takamune T Saito, Monica P Colaiácovo
Oct 5, 2018·Critical Reviews in Biochemistry and Molecular Biology·Jean-Hugues Guervilly, Pierre Henri Gaillard
Jul 23, 2013·PLoS Genetics·Stephanie P Bellendir, Jeff Sekelsky
Oct 22, 2016·G3 : Genes - Genomes - Genetics·T Brooke McClendonJudith L Yanowitz
Nov 26, 2019·FEBS Letters·Matthew CharmanMatthew D Weitzman
Aug 10, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Talia Hatkevich, Jeff Sekelsky

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
two-hybrid
restriction digests
PCR

Software Mentioned

Pfam
COIL
HHpred
SORT
Ensembl

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.