Caffeic acid phenethyl ester reduces the secretion of vascular endothelial growth factor through the inhibition of the ROS, PI3K and HIF-1α signaling pathways in human retinal pigment epithelial cells under hypoxic conditions

International Journal of Molecular Medicine
Sung Hwa PaengIl-Whan Choi

Abstract

Choroidal neovascularization (CNV) can lead to progressive and severe visual loss. Vascular endothelial growth factor (VEGF) promotes the development of CNV. Caffeic acid phenethyl ester (CAPE), a biologically active component of the honeybee (Apis mellifera) propolis, has been demonstrated to have several interesting biological regulatory properties. The objective of this study was to determine whether treatment with CAPE results in the inhibition of the production of vascular endothelial growth factor (VEGF) in retinal pigment epithelial cells (RPE cells) under hypoxic conditions and to explore the possible underlying mechanisms. An in vitro experimental model of hypoxia was used to mimic an ischemic microenvironment for the RPE cells. Human RPE cells (ARPE-19) were exposed to hypoxia with or without CAPE pre-treatment. ARPE-19 cells were used to investigate the pathway involved in the regulation of VEGF production under hypoxic conditions, based on western blot analysis, enzyme-linked immunosorbent assay (ELISA) and electrophoretic mobility shift assay (EMSA). The amount of VEGF released from the hypoxia-exposed cells was significantly higher than that of the normoxic controls. Pre-treatment with CAPE suppressed the hypoxia-...Continue Reading

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Citations

May 20, 2015·International Journal of Molecular Sciences·Ying-Yu KuoChih-Pin Chuu
Sep 17, 2017·Oncotarget·Zhi-Hua OuyangGuo-Hua Lv
Nov 22, 2017·Oxidative Medicine and Cellular Longevity·Valeria PittalàClaudio Bucolo
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Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Clarisse CornebiseDominique Delmas
Dec 22, 2020·Frontiers in Physiology·Henrique Silva, Nuno Miguel F Lopes
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Xiaohuan ZhaoXiaodong Sun
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Batoryna OlgierdMorawiec Emilia

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