PMID: 8944657Nov 1, 1996Paper

Caffeine- and carbachol-induced Cl- and cation currents in single opossum esophageal circular muscle cells

The American Journal of Physiology
Q WangR K Goyal

Abstract

Cl- and cation currents may play important roles in esophageal smooth muscle membrane potential changes and contraction. We studied Ca2+ release-activated cell-shortening and membrane currents in single cells freshly dispersed from the circular muscle of the opossum esophagus using the standard patch-clamp whole cell recording method. Caffeine (10-20 microM) and carbachol (10-100 microM) shortened the single smooth muscle cells by releasing intracellular Ca2+. At a holding potential of 0 mV, spontaneous transient outward currents STOCs, representing spontaneous Ca(2+)-activated K+ currents) were recorded. Caffeine, carbachol, or ionomycin evoked large outward currents (up to 1,650 pA) and subsequently abolished STOCs. At a holding potential of -50 mV in K(+)-containing solutions, an outward current in response to the agonists was observed; in some cells, the outward current followed an inward current. In K(+)-free solutions, the agonists induced only an inward current whose reversal potential was shifted by alteration of the anion gradient but not by that of the cation. With a low-Cl- pipette solution (Cl- substituted by glucuronate or glutamate), the inward currents were dependent mainly on the external cation gradient. This c...Continue Reading

Citations

Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
Jun 2, 2011·Pediatric Surgery International·Tutku SoyerMurat Cakmak
Sep 3, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco GhionzoliPaolo De Coppi
May 9, 2007·Annals of Biomedical Engineering·Alberto Corrias, Martin L Buist
Apr 28, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xue-Dao He, Raj K Goyal
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Maneesh A DeshpandeStephen M Sims
Nov 14, 1998·The American Journal of Physiology·R K Goyal, X D He

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