PMID: 8584440Jan 1, 1996Paper

Caffeine enhancement of electrical activity through direct blockade of inward rectifying K+ currents in GH3 rat anterior pituitary cells

Pflügers Archiv : European journal of physiology
F BarrosP de la Peña

Abstract

Treatment of rat anterior pituitary GH3 cells with caffeine causes a reversible enhancement of electrical activity superimposed over a depolarization of the plasma membrane potential. Similar results are obtained with theophylline, but not with isobutylmethylxanthine or forskolin. The effects of caffeine are not related to Ca2+ liberation from intracellular stores since they are not affected by incubation of the cells with ryanodine or thapsigargin. Furthermore, caffeine-induced hyperpolarization of the membrane is not detectable even in cells in which Ca2+ liberation from inositol 1,4,5-trisphosphate-sensitive compartments produces a prominent transient hyperpolarization in response to thyrotropin-releasing hormone. Reductions of Ca2+-dependent K+ currents caused by partial block of L-type Ca2+ channels by caffeine are not sufficient to explain the effects of the xanthine, since the results obtained with caffeine are not mimicked by direct blockade of Ca2+ channels with nisoldipine. GH3 cell inwardly rectifying K+ currents are inhibited by caffeine. Studies on the voltage dependence of the caffeine-induced effects indicate a close correlation between alterations of electrical parameters and reported values of steady-state volt...Continue Reading

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Citations

Aug 4, 2004·Progress in Biophysics and Molecular Biology·William BainsCat White
Dec 12, 2012·PloS One·Arne Bilet, Christiane K Bauer
Aug 11, 2016·Biophysical Journal·Maike Mauerhöfer, Christiane K Bauer
Apr 1, 1997·Journal of Cellular Physiology·L Karhapää, K Törnquist
Oct 7, 2005·American Journal of Physiology. Cell Physiology·Agnese SecondoLucio Annunziato
Feb 6, 2007·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Yoshitaka Fujii, Masahiro Nakayama
Feb 17, 2007·American Journal of Physiology. Cell Physiology·Xia MaoYu-Fung Lin
Oct 27, 1999·Canadian Journal of Physiology and Pharmacology·A PetitB T Lussier
Oct 18, 2005·The Journal of Pharmacology and Experimental Therapeutics·S L Cockerill, J S Mitcheson
Feb 8, 2002·American Journal of Physiology. Cell Physiology·James D JohnsonJohn P Chang

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