Caffeine-induced Ca(2+) signaling as an index of cardiac progenitor cells differentiation

Basic Research in Cardiology
C AltomareAntonio Zaza

Abstract

Cardiac progenitor cells (CPCs), migrating from heart tissue, in culture aggregate to form cardiospheres (CSs) in which replication and cardiogenic differentiation occur. However, the frequency of functional differentiation in CSs and the role of cell clustering in supporting it remain to be established. The aim of our study is to quantify differentiation of a muscle-type Ca(2+) release mechanism in CS-derived cells, correlate it with cardiac differentiation markers and test its dependency on CS formation. CPCs migrating from murine cardiac explants were studied prior and after CSs formation (Pre-CS and Post-CS). Inducibility of RyR- and IP3-R-mediated Ca(2+) transients in individual cells was tested by exposure to caffeine and ATP, respectively; expression of cardiac and non-cardiac lineage markers was assessed. Caffeine responsiveness was negligible in Pre-CS cells and increased by 7.5 fold in Post-CS cells (3.6 vs. 26.9%; p < 0.05), and was closely correlated with activation of the cardiac TnI gene promoter. ATP-induced responses, frequent in Pre-CS (86%), were slightly increased in Post-CS cells (94%; p < 0.05). Expression of cardiac-specific Ca(2+)-handling proteins (Cav1.2, NCX1, RyR2, SERCA2a) was either limited to the P...Continue Reading

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Citations

Feb 8, 2013·Journal of Cellular and Molecular Medicine·Claudia AltomareAntonio Zaza
Nov 22, 2011·Journal of Cellular and Molecular Medicine·Lucio Barile, Vincenzo Lionetti
Jan 22, 2014·Journal of Cellular and Molecular Medicine·Isotta ChimentiAlessandro Giacomello
Jun 15, 2013·Stem Cells International·Lucio BarileGiuseppe Vassalli
Aug 28, 2012·Biochimica Et Biophysica Acta·Isotta ChimentiAlessandro Giacomello
Jun 25, 2016·BioImpacts : BI·Francesco AngeliniAlessandro Giacomello
Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Manuela CampanFabio A Recchia
Oct 12, 2011·Expert Review of Cardiovascular Therapy·Lucio BarileAntonio Zaza
Sep 1, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Enea PianezziGiuseppe Vassalli

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