PMID: 8584430Jan 1, 1996Paper

Caffeine-induced oscillations of cytosolic Ca2+ in GH3 pituitary cells are not due to Ca2+ release from intracellular stores but to enhanced Ca2+ influx through voltage-gated Ca2+ channels

Pflügers Archiv : European journal of physiology
C Villalobos, Javier García-Sancho

Abstract

Caffeine, a well known facilitator of Ca2+-induced Ca2+ release, induced oscillations of cytosolic free Ca2+ ([Ca2+]i) in GH3 pituitary cells. These oscillations were dependent on the presence of extracellular Ca2+ and blocked by dihydropyridines, suggesting that they are due to Ca2+ entry through L-type Ca2+ channels, rather than to Ca2+ release from the intracellular Ca2+ stores. Emptying the stores by treatment with ionomycin or thapsigargin did not prevent the caffeine-induced [Ca2+]i oscillations. Treatment with caffeine occluded phase 2 ([Ca2+]i oscillations) of the action of thyrotropin-releasing hormone (TRH) without modifying phase 1 (Ca2+ release from the intracellular stores). Caffeine also inhibited the [Ca2+]i increase induced by depolarization with high-K+ solutions (56% at 20 mM), suggesting direct inhibition of the Ca2+ entry through voltage-gated Ca2+ channels. We propose that the [Ca2+]i increase induced by caffeine in GH3 cells takes place by a mechanism similar to that of TRH, i.e. membrane depolarization that increases the firing frequency of action potentials. The increase of the electrical activity overcomes the direct inhibitory effect on voltage-gated Ca2+ channels with the result of increased Ca2+ entr...Continue Reading

References

Jun 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C VillalobosJ García-Sancho
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Citations

Jan 16, 2003·Cell Calcium·Maria Teresa AlonsoJavier García-Sancho
Oct 24, 2001·European Journal of Pharmacology·H YamamotoT Yamamoto
Jan 29, 1999·The Journal of Cell Biology·M T AlonsoJ Alvarez
Jun 23, 2007·Cell Calcium·Pablo ChameroJavier García-Sancho
Nov 15, 2002·The European Journal of Neuroscience·Ricardo RigualJavier Alvarez
Apr 1, 1997·Journal of Cellular Physiology·L Karhapää, K Törnquist
Oct 23, 2001·The Journal of Biological Chemistry·C VillalobosJ García-Sancho
Nov 13, 2002·The Journal of Biological Chemistry·Pablo ChameroJavier García-Sancho
Jun 5, 1998·The American Journal of Physiology·L Nuñez, L S Frawley
Jan 22, 1998·The American Journal of Physiology·C VillalobosJ García-Sancho

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