PMID: 9448945Feb 4, 1998Paper

Caffeine mediates cation influx and intracellular Ca2+ release in leech P neurones

Cell Calcium
Jürgen SchoppeW R Schlue

Abstract

We investigated the effect of caffeine on the intracellular free Ca2+ concentration ([Ca2+]i) of leech P neurones by using the fluorescent indicator Fura-2. Caffeine induced a [Ca2+]i increase that was strongly reduced, but not abolished, in Ca(2+)-free solution. The effect of caffeine on [Ca2+]i was dose-dependent: while 5 mM caffeine evoked a persistent [Ca2+]i increase that could be elicited repetitively, 10 mM caffeine or more induced a transient [Ca2+]i increase that was strongly reduced upon subsequent applications at the same concentration. Surprisingly, the cells remained fully responsive to a moderately increased caffeine concentration. The caffeine-induced [Ca2+]i increase was not blocked by millimolar concentrations of La3+, Mg2+, Cd2+, Zn2+, Co2+, Ni2+, or Mn2+. While La3+ and Mg2+ had no effect on the caffeine response, the other cations caused irreversible changes in the Fura-2 fluorescence. The inhibitors of intracellular Ca2+ pumps-thapsigargin, cyclopiazonic acid (CPA), and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ)--had no effect on the caffeine-induced [Ca2+]i increase at normal extracellular Ca2+ concentration, but they reduced it in Ca(2+)-free solution. Ryanodine had no effect on the caffeine-induced [Ca2+]i ...Continue Reading

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Citations

Jul 6, 2002·Cell Calcium·J F Cordero, P J Romero
Feb 10, 2004·Biology of Reproduction·Becky Marquez, Susan S Suarez
Oct 23, 2009·International Journal of Andrology·C ColásT Muiño-Blanco
Apr 6, 2006·The Journal of Membrane Biology·G KleesP W Dierkes
May 8, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Yu-An ZhangHui Zou
Aug 29, 2012·Journal of Muscle Research and Cell Motility·Shuzhe DingMichael R Duchen
Feb 26, 2020·Journal of Neuroscience Research·Oliva Mota SeguraMichael W Baker
Oct 2, 2020·Animal Reproduction Science·Eduardo de MercadoEmilio Gómez-Izquierdo
Jun 15, 2004·Brain Research·Paul Wilhelm DierkesWolf-Rüdiger Schlue

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