Caffeine prevents hyperoxia-induced lung injury in neonatal mice through NLRP3 inflammasome and NF-κB pathway.

Respiratory Research
Shangqin ChenLizhong Du

Abstract

Bronchopulmonary dysplasia (BPD) is a common chronic lung disease in premature infants and hyperoxia exposure is a major cause. In hyperoxic lung injury animal model, alveolar simplification and pro-inflammatory cells infiltration are the main pathophysiologic changes. Caffeine is a drug used to treat apnea in premature infants. Early use of caffeine can decrease the rate and the severity of BPD while the mechanisms are still unclear. The purpose of this study was to evaluate the effects of caffeine on inflammation and lung development in neonatal mice with hyperoxic lung injury and to explore the possible mechanism. Following 14 d of 75% oxygen exposure in newborn mouse, the BPD model was established. Caffeine at a dose of 1 g/L was added in drinking water to nursing mouse. We measured the concentration of caffeine in serum and oxidative stress in lung by commercially available kits. Adenosine 2A receptor (A2AR) expression and lung inflammation were measured by Immunohistochemistry and western blotting. Apoptosis and surfactant protein-C (SFTPC) levels were measured by immunofluorescence. The inflammasome and NF-κB pathway proteins were assessed by western blotting. We found that the caffeine concentration in plasma at present...Continue Reading

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Citations

Feb 23, 2021·Frontiers in Pharmacology·Bohao LiuQing Geng
Aug 4, 2021·International Immunopharmacology·Ramazan OzdemirMehmet Aslan
Aug 7, 2021·Journal of Perinatology : Official Journal of the California Perinatal Association·Shilpa Vyas-ReadJoanne M Lagatta
Sep 2, 2021·Clinical & Translational Immunology·Lakshanie C WickramasingheMargaret L Hibbs

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
MDA
Protein Assay

Software Mentioned

GraphPad
SPSS

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