Caffeine-sensitive calcium stores in presynaptic nerve endings: a physiological role?

Biochemical and Biophysical Research Communications
A Martínez-Serrano, J Satrústegui

Abstract

Ca2+-sensitive minielectrodes and the fluorescent cytosolic calcium probes, quin2 and fura2, were used to study some aspects of calcium homeostasis in intact and permeabilized synaptosomes from whole rat brain. Experiments in permeabilized synaptosomes revealed the existence of a vesicular, non-mitochondrial, ATP-dependent calcium uptake system with a vanadate sensitivity similar to that of brain microsomes, or endoplasmic reticulum-type calcium sequestering organelles. By using the fluorescent probes it was possible to show that caffeine mobilizes calcium from these internal stores in intact synaptosomes. Our results indicate a role of the caffeine sensitive calcium stores in the buffering of calcium loads elicited by plasma membrane depolarization.

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Citations

Mar 7, 1996·European Journal of Pharmacology·R A PaduaJ D Geiger
Oct 1, 1993·Neurochemistry International·E TareilusH Breer
Nov 1, 1994·Neuroscience·P Kostyuk, A Verkhratsky
Jan 1, 1993·Journal of Physiology, Paris·J VautrinJ L Barker
Jun 5, 2002·Neuroscience Letters·Saleh Al-DeebMohammad Tariq
Feb 1, 1992·Molecular Biology of the Cell·A Martínez-Serrano, J Satrústegui
Sep 13, 1991·Biochimica Et Biophysica Acta·H T McMahon, D G Nicholls

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