PMID: 8962108Dec 10, 1996Paper

cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors

Proceedings of the National Academy of Sciences of the United States of America
S CensiniA Covacci

Abstract

cagA, a gene that codes for an immunodominant antigen, is present only in Helicobacter pylori strains that are associated with severe forms of gastroduodenal disease (type I strains). We found that the genetic locus that contains cagA (cag) is part of a 40-kb DNA insertion that likely was acquired horizontally and integrated into the chromosomal glutamate racemase gene. This pathogenicity island is flanked by direct repeats of 31 bp. In some strains, cag is split into a right segment (cagI) and a left segment (cagII) by a novel insertion sequence (IS605). In a minority of H. pylori strains, cagI and cagII are separated by an intervening chromosomal sequence. Nucleotide sequencing of the 23,508 base pairs that form the cagI region and the extreme 3' end of the cagII region reveals the presence of 19 ORFs that code for proteins predicted to be mostly membrane associated with one gene (cagE), which is similar to the toxin-secretion gene of Bordetella pertussis, ptlC, and the transport systems required for plasmid transfer, including the virB4 gene of Agrobacterium tumefaciens. Transposon inactivation of several of the cagI genes abolishes induction of IL-8 expression in gastric epithelial cell lines. Thus, we believe the cag regio...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Galán, R Curtiss
Dec 1, 1981·Journal of Bacteriology·D A Portnoy, S Falkow
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LiR K Selander
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Oct 1, 1994·Journal of Clinical Pathology·J E CrabtreeD S Tompkins
Nov 11, 1994·Nucleic Acids Research·M BorodovskyE V Koonin
May 5, 1994·The New England Journal of Medicine·J ParsonnetG D Friedman
May 1, 1994·The Journal of Experimental Medicine·J L TelfordZ Xiang
Jan 1, 1993·Scandinavian Journal of Immunology·J E CrabtreeI J Lindley
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E A GroismanH Ochman
Jan 1, 1993·Trends in Biochemical Sciences·G P Salmond, P J Reeves
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A A WeissD L Burns
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A CovacciN Figura
Jan 1, 1995·Methods in Enzymology·N Lisitsyn, M Wigler
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E SheaD W Holden
Feb 1, 1996·Trends in Microbiology·S C WinansP J Christie

❮ Previous
Next ❯

Citations

Jul 22, 1999·Journal of Clinical Laboratory Analysis·D BassoM Plebani
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Young-Woon ChangRin Chang
Mar 1, 2000·Biochemical and Biophysical Research Communications·R PalovuoriT J Karttunen
Feb 17, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Mitsushige Sugimoto, Yoshio Yamaoka
May 23, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Aneta TargoszWieslaw Pawlik
May 14, 2009·Archives of Microbiology·M Victoria DelpinoVito G Delvecchio
Jun 19, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A R PachecoM Brocchi
Dec 18, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W BartchewskyM L Ribeiro
Feb 17, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H-M A SchmidtH Mitchell
Apr 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L T NguyenM Moriyama
Jan 21, 2011·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Hideyuki UbukataTakafumi Tabuchi
Jan 5, 2005·Digestive Diseases and Sciences·Andrew S DayPhilip M Sherman
Oct 22, 2005·Digestive Diseases and Sciences·Farideh SiavoshiHassan Ashktorab
Mar 8, 2007·Digestive Diseases and Sciences·Kee Don ChoiIn Sung Song
Nov 21, 2002·Current Gastroenterology Reports·Pelayo Correa
Dec 4, 2003·Journal of Molecular Biology·Anna ZawilakJolanta Zakrzewska-Czerwińska
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ian M CarrollNiyaz Ahmed
Aug 3, 2001·Primary Care·D T SmootB Cryer
Oct 28, 1998·Molecular Aspects of Medicine·G Del GiudiceR Rappuoli
Oct 2, 2002·Lancet·Francis K L Chan, W K Leung
Oct 29, 2002·Journal of Microbiological Methods·Ramon de JongeJohannes G Kusters
Nov 11, 2003·Journal of Microbiological Methods·Liviu A SicinschiBarbara G Schneider
Feb 17, 2001·Archives of Medical Research·J TorresO Muñoz
Oct 13, 2000·Gastroenterology Clinics of North America·H M Windsor, J O'Rourke

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.