Abstract
Cytotoxin-associated antigen A (CagA), a major virulence factor of Helicobacter pylori (Hp), is associated with the pathogenesis of peptic ulcer and gastric cancer. Recent researches demonstrated that Hp exists in palatine tonsil in all studied IgA nephropathy (IgAN) patients, most of which were CagA-positive, suggesting that CagA may be a causative pathogenic factor of IgAN. However, the underlying molecular mechanisms and signaling pathway are still largely unclear. In the present study, CCK8 assay, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to investigate the effect of CagA on cell proliferation and extracellular matrix secretion in rat glomerular mesangial cells. RT-PCR and western blotting were used to reveal the potential signaling pathway. Rat glomerular mesangial cells were treated with recombinant CagA protein for 72 h, in a dose- and time-dependent manner. We found that CagA promoted cell proliferation and extracellular matrix secretion by inhibiting signaling pathway of apoptosis. Taken together, these findings suggested that CagA induced cellular injury in glomerular mesangium by proliferation and secretion of extracellular matrix, and may play an important role in pathogenesis of IgAN.
References
May 26, 1990·Lancet·E A Rauws, G N Tytgat
Jun 1, 1997·Biochemical and Molecular Medicine·W ZhangN H Ansari
Nov 22, 1997·Virchows Archiv : an International Journal of Pathology·R NagashimaT Takahashi
Jul 8, 1998·American Journal of Nephrology·S UdaT Ideura
Jun 3, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J BarrattA C Allen
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·E D SegalL S Tompkins
May 31, 2003·Science·Manuel R AmievaStanley Falkow
May 22, 2007·Life Sciences·Rong WangJing Sun
Aug 24, 2007·Human Pathology·Kenichiro KusanoAkira Inokuchi
Dec 10, 2009·Journal of Gastroenterology·Kenichiro KusanoJunji Miyazaki
May 22, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rosanna Coppo
Oct 12, 2010·Clinical Microbiology Reviews·Lydia E WroblewskiKeith T Wilson
Dec 29, 2010·Clinical Reviews in Allergy & Immunology·Hsin-Hui YuBor-Luen Chiang
Sep 29, 2011·Journal of the American Society of Nephrology : JASN·Hitoshi SuzukiBruce A Julian
Oct 12, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alain P GobertKeith T Wilson
Feb 10, 2012·Kidney International·Joanna K BoydJonathan Barratt
Mar 21, 2012·Nature Reviews. Nephrology·Kar Neng Lai
Jun 21, 2013·The New England Journal of Medicine·Robert J Wyatt, Bruce A Julian
Jan 28, 2014·Biochemical and Biophysical Research Communications·Man YangJun-ming Fan
Mar 19, 2014·Acta Pharmacologica Sinica·Nan MaoJun-ming Fan
Jun 4, 2014·The Journal of Clinical Investigation·Krzysztof Kiryluk, Jan Novak
Aug 30, 2014·Helicobacter·Leonardo H EusebiFranco Bazzoli
Dec 3, 2014·Best Practice & Research. Clinical Gastroenterology·Rui M FerreiraCeu Figueiredo
Citations
Mar 31, 2005·American Journal of Hypertension·Sol M BobstRodney E Kellems
Mar 10, 2017·Gut and Liver·Uday C GhoshalUjjala Ghoshal
Jun 12, 2020·Renal Failure·Xing-Zi LiuHong Zhang
Jul 19, 2019·Experimental and Therapeutic Medicine·Dongmei ZhangWanxin Tang
Feb 27, 2017·Cellular and Molecular Life Sciences : CMLS·Trevor W Stone, L Gail Darlington
Apr 4, 2021·International Journal of Molecular Sciences·Agnieszka KrupaMagdalena Chmiela