Caged siRNAs with single folic acid modification of antisense RNA for photomodulation of exogenous and endogenous gene expression in cells

Organic & Biomolecular Chemistry
Lijia YuXinjing Tang

Abstract

Manually controlling siRNA activity is an essentially important way to spatiotemporally investigate gene expression and function. Owing to ease of operation and precise manipulation, light can be used for controlled regulation of siRNA-induced gene silencing. Here, we developed a series of caged siRNAs with folic acid modification at the 5' terminus of the antisense strand of the siRNA through a photolabile linker. The attachment of the folic acid moiety temporarily masked the corresponding siRNA activity. Upon illumination, these caged siRNAs were activated, and their gene silencing activities were restored. Based on this strategy, we successfully photomodulated gene expression of both an exogenous gene (for green fluorescent protein, GFP) and an endogenous gene (for mototic kinesin-5, Eg5) in cells.

References

Jan 1, 1993·Methods in Molecular Biology·M J Damha, K K Ogilvie
Jul 19, 2001·Bioconjugate Chemistry·D A Stetsenko, M J Gait
Sep 21, 2001·The Journal of Cell Biology·T M Kapoor, T J Mitchison
Apr 25, 2002·Journal of the American Chemical Society·Richard P Fahlman, Dipankar Sen
Apr 20, 2004·Advanced Drug Delivery Reviews·Shefali Sabharanjak, Satyajit Mayor
May 19, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiyuki Hattori, Yoshie Maitani
Nov 13, 2004·Nature·Jürgen SoutschekHans-Peter Vornlocher
Jan 12, 2005·Angewandte Chemie·Samit ShahSimon H Friedman
Feb 25, 2006·Biochimica Et Biophysica Acta·Quan N NguyenGary K McMaster
Oct 24, 2007·RNA·Vera Mikat, Alexander Heckel
Dec 7, 2007·Nucleic Acids Research·XinJing TangIvan J Dmochowski
Oct 3, 2009·Annals of the New York Academy of Sciences·Mini ThomasPhilip S Low
Dec 18, 2010·Journal of the American Chemical Society·Piyush K JainSimon H Friedman
Jun 1, 2012·Journal of the American Chemical Society·Ran XieXing Chen
Sep 5, 2012·Molecular BioSystems·Colleen M ConnellyAlexander Deiters
Jan 25, 2013·Molecular Therapy. Nucleic Acids·Christian DohmenErnst Wagner
Sep 12, 2013·Nucleic Acids Research·Jeane M GovanAlexander Deiters
Mar 29, 2014·International Journal of Molecular Sciences·Cui HuJian-Hui Jiang
Apr 14, 2015·Journal of Inorganic Biochemistry·Brittani K RubleIvan J Dmochowski
Oct 17, 2015·Methods in Molecular Biology·Thomas C RobertsMarc S Weinberg
Jan 7, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Eugénia NogueiraArtur Cavaco-Paulo
Mar 25, 2016·Journal of the American Chemical Society·Lisa ShahIsaac S Carrico
May 19, 2017·Angewandte Chemie·Nicholas AnkenbruckAlexander Deiters
Jan 10, 2018·Medicinal Research Reviews·Changmai ChenXinjing Tang
Mar 1, 2018·Chembiochem : a European Journal of Chemical Biology·Liangliang ZhangXinjing Tang
Mar 10, 2018·Beilstein Journal of Organic Chemistry·Françoise DebartJean-Jacques Vasseur

❮ Previous
Next ❯

Citations

Jul 14, 2020·Biochemical Society Transactions·Denis HartmannMichael J Booth
Jul 18, 2020·Chemistry : a European Journal·Jinhao ZhangXinJing Tang
Jan 13, 2021·Chembiochem : a European Journal of Chemical Biology·Qian WangXinjing Tang
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Linlin Yang, Ivan J Dmochowski
Nov 6, 2021·Chemical Society Reviews·Kristie E Darrah, Alexander Deiters

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
flow cytometry
flow
transfection
fluorescence microscopy
PCR
confocal microscopy
X-ray

Software Mentioned

ModFitLT
CytExpert

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.