Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: basal values determination of micronucleus and comet assay

Mutation Research
G L PolettaMarta Mudry

Abstract

Caiman latirostris is one of the two crocodilian species that inhabit Argentina. In this country, as a consequence of agricultural frontiers expansion during the last years, many areas of the geographic distribution of the broad snouted caiman overlap with regions of intensive agricultural activity. Contaminants released to the environment may induce genetic alterations in wildlife, which could lead to mutations and/or carcinogenesis. Up to the moment, no studies had been made concerning the possibbility to apply biomarkers of genotoxic evaluation in C. latirostris. The aim of this study was to adapt two widely used genotoxic techniques, the comet assay and the micronucleus test, for their application in C. latirostris and to determine the baseline values in this species, in order to establish its suitability as a sentinel organism for future genotoxic monitoring of environmental pollutants. A total of 41 juvenile caimans of 4 months old (FMO) and 10 months old (TMO) were used. Genotoxic techniques were applied on peripheral blood erythrocytes introducing the necessary modifications required by the material, which are presented here. Our results show that baseline values of DNA damage are quite stable among juvenile caimans (MN...Continue Reading

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Citations

Oct 12, 2013·Marine Environmental Research·Ilaria CalianiMaria Cristina Fossi
Apr 1, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Laura G SchaumburgMarta D Mudry
Aug 21, 2012·Ecotoxicology and Environmental Safety·Laura G SchaumburgMarta D Mudry
Jan 19, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Luis Francisco RossiMarta Dolores Mudry
Jun 23, 2009·Toxicology·Céline GasnierGilles-Eric Séralini
Aug 25, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Gisela L PolettaMarta D Mudry
Oct 12, 2013·Environmental Toxicology and Chemistry·Paul B C GrantPeter S Ross
Oct 1, 2016·Environmental Science and Pollution Research International·Arnoldo A M QueroNora B M Gorla
Oct 25, 2016·Archives of Environmental Contamination and Toxicology·Pamela Mariana BurellaGisela Laura Poletta
Nov 4, 2016·EcoHealth·Jonathan Pérez-FloresDaniel Atilano
Mar 11, 2020·Biological Reviews of the Cambridge Philosophical Society·Ruchira SomaweeraBruce L Webber
Aug 27, 2019·Biological Reviews of the Cambridge Philosophical Society·Thiago F RodriguesAdriano G Chiarello
Jun 26, 2020·Environmental Monitoring and Assessment·Jadna Maria SilvaEliza Maria Xavier Freire
Dec 12, 2020·Environmental Toxicology and Pharmacology·Omar Cruz-SantiagoCésar Arturo Ilizaliturri-Hernández
Aug 14, 2020·The Science of the Total Environment·José Silonardo Pereira de OliveiraGuilherme Malafaia
Jul 17, 2021·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Camila Felisa ChacónGisela Laura Poletta

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