Cajuputs candy impairs Candida albicans and Streptococcus mutans mixed biofilm formation in vitro

F1000Research
Siska SeptianaChristofora Hanny Wijaya

Abstract

Background:Cajuputs candy (CC), an Indonesian functional food, utilizes the bioactivity of Melaleuca cajuputi essential oil (MCEO) to maintain oral cavity health. Synergistic interaction between Candida albicans and Streptococcus mutans is a crucial step in the pathogenesis of early childhood caries. Our recent study revealed several alternative MCEOs as the main flavors in CC. The capacity of CC to interfere with the fungus-bacterium relationship remains unknown. This study aimed to evaluate CC efficacy to impair biofilm formation by these dual cariogenic microbes. Methods: The inhibition capacity of CC against mixed-biofilm comprising C. albicans and S. mutans was assessed by quantitative (crystal violet assay, tetrazolium salt [MTT] assay, colony forming unit/mL counting, biofilm-related gene expression) and qualitative analysis (light microscopy and scanning electron microscopy). Result: Both biofilm-biomass and viable cells were significantly reduced in the presence of CC. Scanning electron microscopy imaging confirmed this inhibition capacity, demonstrating morphology alteration of C. albicans, along with reduced microcolonies of S. mutans in the biofilm mass. This finding was related to the transcription level of selecte...Continue Reading

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Jun 17, 2020·F1000Research·Siska SeptianaChristofora Hanny Wijaya

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Jun 17, 2020·F1000Research·Siska SeptianaChristofora Hanny Wijaya

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Software Mentioned

GraphPad
SPSS Statistics
GraphPad Prism

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