Calbindin-D28k and calretinin immunoreactivity in the spinal cord of the lizard Gekko gecko: Colocalization with choline acetyltransferase and nitric oxide synthase

Brain Research Bulletin
Ruth MoronaAgustín González

Abstract

The distribution of the calcium-binding proteins calbindin-D28k (CB) and calretinin (CR) was investigated in the spinal cord of the lizard Gekko gecko, by means of immunohistochemical techniques. Abundant cell bodies and fibers immunoreactive for either CB or CR were widely distributed throughout the spinal cord. Most neurons and fibers were labeled in the superficial dorsal horn, but numerous cells were also located in the intermediate gray and ventral horn. Distinct CB- and CR-containing cell populations were observed, although double immunohistochemistry revealed that 17-20% of the single-labeled cells for CB or CR in the dorsal horn contained both proteins. In addition, nitric oxide synthase was immunodetected in about 6% of the CB-positive neurons in the dorsal horn and in 10% in the ventral horn, whereas nitric oxide synthase was present in 9-13% of CR-positive cells in the dorsal horn and in 14% in the ventral horn. These doubly immunoreactive cells were restricted to areas IV, VII and VIII. Similar colocalization experiments revealed that 18-24% of the cholinergic cells in the ventral horn contained CB and 21-30% CR, with some variations throughout the length of the spinal cord. The pattern of distribution for CB and CR...Continue Reading

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Citations

Mar 12, 2008·Brain Research Bulletin·Amaia ViloriaManuel Megías
May 30, 2009·The Journal of Comparative Neurology·Ruth Morona, Agustin González
May 24, 2014·Journal of Molecular Neuroscience : MN·Judyta K Juranek, Joanna A Wojtkiewicz
Aug 20, 2016·The Journal of Comparative Neurology·Ruth MoronaAgustín González
Nov 6, 2010·Brain, Behavior and Evolution·Ruth MoronaAgustín González
Mar 10, 2018·The Journal of Comparative Neurology·Ruth MoronaAgustín González

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