Calbindin-D28k decreases L-type calcium channel activity and modulates intracellular calcium homeostasis in response to K+ depolarization in a rat beta cell line RINr1046-38

Cell Calcium
David LeeSylvia Christakos

Abstract

Calbindin-D(28k), acts as a modulator of depolarization induced calcium transients in the pancreatic beta cell. However, specific mechanisms have not been defined. Here we show for the first time that the calcium binding protein calbindin-D(28k) acts by affecting calcium influx through voltage-dependent calcium channels in RIN pancreatic beta cells. Whole-cell patch-clamp recordings revealed that Ca(2+) current amplitudes of calbindin-D(28k) expressing RINr1046-38 beta cells were smaller than the Ca(2+) current amplitudes in control cells in response to depolarizing pulses. The peak current was observed at +20mV and the average amplitude was approximately 50pA in the calbindin expressing cells compared to approximately 250pA in control cells. In calbindin-D(28k) expressing cells, the channels had enhanced sensitivity to Ca(2+) dependent inactivation and currents decayed much more rapidly than in control cells. The Ca(2+) channels affected by calbindin were found to have biophysical properties consistent with dihydropyridine-sensitive L-type calcium channels. In response to depolarizing concentrations of K(+), calbindin expression caused a five-fold decrease in the rate of rise of [Ca(2+)](i) and decay was slower in the calbindi...Continue Reading

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Citations

Mar 9, 2011·The Journal of Biological Chemistry·Shimrit OzNathan Dascal
Jun 22, 2006·Acta Pharmacologica Sinica·Lynn McKeownOwen T Jones
Jan 30, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Sylvia ChristakosQi Shen
Jan 5, 2008·The Journal of Physiology·Stuart L Johnson, Walter Marcotti
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Apr 21, 2007·Annals of the New York Academy of Sciences·Suk-Ho LeeWon-Kyung Ho
Aug 12, 2015·Biochemical and Biophysical Research Communications·Asghar HajibeigiOrhan K Öz
Jan 10, 2012·Autonomic Neuroscience : Basic & Clinical·Petr M MasliukovJean-Pierre Timmermans
Sep 3, 2014·PloS One·Venkatraman SiddharthanJohn D Morrey
Aug 29, 2018·International Journal of Molecular Sciences·Julien B PujolEl Hadji M Dioum
Jun 22, 2019·The Journal of Physiological Sciences : JPS·Kannikar WongdeeNarattaphol Charoenphandhu
Aug 24, 2011·Reviews in Endocrine & Metabolic Disorders·Sylvia Christakos

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