Calbindin-D9K immunolocalization and vitamin D-dependence in the bone of growing and adult rats

Histochemistry
N BalmainH Mathieu

Abstract

This report presents evidence for the presence of the vitamin D-dependent calcium-binding protein, calbindin-D9K, in bone cells and matrix. In undecalcified frozen sections of growing and adult rat bone, calbindin-D9K was immunohistochemically localized in trabecular bone of the epiphysis and metaphysis and in cortical bone of the diaphysis. It was found within the cytoplasm of osteocytes, of osteoblasts lining the osteoid, and osteoblasts inside the osteoid seams. It was also found in the osteoblast processes and the anastomosed reticulum of the processes connecting the osteocytes with each other. Extracellularly, calbindin-D9K immunoreactivity was present in compact cortical bone in the areas of the mineralized matrix surrounding the osteocyte lacunae, and in the pericanalicular walls containing the cell processes. Calbindin-D9K immunoreactivity was low or absent from the cytoplasm of osteocytes in trabecular bone from severely vitamin D-deficient rats and restored in vitamin D-deficient rats given a single dose of 1,25(OH)2-VitD3. Thus, the synthesis of immunoreactive calbindin-D9K by osteoblasts and osteocytes in trabecular bone is vitamin D-dependent. The presence of immunoreactive calbindin-D9K in the osteocytes and their...Continue Reading

References

Dec 8, 1978·Calcified Tissue Research·E H Burger, J L Matthews
Jan 1, 1979·The Journal of Endocrinology·S C ManolagasD C Anderson
Jun 20, 1977·Biochemical and Biophysical Research Communications·P MarcheM Thomasset
Aug 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N BalmainH Mathieu
Sep 1, 1988·Calcified Tissue International·F Bronner
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y S LeeR H Wasserman
Aug 1, 1985·The Anatomical Record·T A CurtisD F Weber
Aug 11, 1969·Calcified Tissue Research·L F Bélanger
Jul 31, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·M A PreeceE Kodicek
Jan 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T A ReinhardtB W Hollis
Mar 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A N Taylor
Jan 1, 1982·Calcified Tissue International·V CanèM A Muglia

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Citations

Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y Mikuni-TakagakiS Saito
Jun 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A BerdalA Nanci
Jan 1, 1991·Histochemistry·N BalmainH Mathieu
Jun 16, 2011·BioFactors·Harry C BlairMone Zaidi
Dec 25, 2004·Physiological Reviews·Joost G J HoenderopRené J M Bindels
May 21, 2011·American Journal of Respiratory and Critical Care Medicine·Scott T Weiss, Augusto A Litonjua
Aug 29, 2020·Bone·Paul H SchlesingerHarry C Blair

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