Calcineurin-dependent lytic granule exocytosis in NK-92 natural killer cells

Cellular Immunology
Arun T Pores-FernandoAdam Zweifach

Abstract

Cytotoxic T cells (CTLs) and natural killer cells (NKs) both kill virus-infected cells and tumor cells by releasing the cytoxic contents of their lytic granules. We recently demonstrated a role for calcineurin in lytic granule exocytosis in TALL-104 human leukemic CTLs [M.J. Grybko, J.P. Bartnik, G.A. Wurth, A.T. Pores-Fernando, A. Zweifach, Calcineurin activation is only one calcium-dependent step in cytotoxic T lymphocyte granule exocytosis, J. Biol. Chem. 282 (2007) 18009-18017]. However, whether calcineurin plays a similar role in NK lytic granule release is not known. We tested whether calcineurin is involved in lytic granule exocytosis in human leukemic NK-92 cells using immunosuppressive drugs that block calcineurin function and by overexpressing a constitutively active calcineurin fusion protein. Our results indicate that calcineurin does play a role in lytic granule exocytosis in NK-92 cells, and suggest that, as was the case in TALL-104 cells, there are likely to be multiple calcium-dependent steps.

References

Feb 1, 1981·Transplantation·M IntronaA Mantovani
Aug 28, 2001·Journal of Hematotherapy & Stem Cell Research·T TonnE Seifried
Oct 3, 2002·Nature Reviews. Immunology·Joseph A Trapani, Mark J Smyth
May 27, 2003·Nature Reviews. Immunology·Judy Lieberman
Nov 2, 2004·Nature Reviews. Immunology·Laurie H GlimcherGraham M Lord
Dec 18, 2004·Journal of Immunological Methods·Galit AlterMarcus Altfeld
Aug 25, 2006·Critical Reviews in Immunology·Sasa RadojaStanislav Vukmanovic
Nov 15, 2006·Immunological Reviews·Yenan T BrycesonEric O Long
May 5, 2007·The Journal of Biological Chemistry·Michael J GrybkoAdam Zweifach

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Citations

Apr 13, 2011·Immunology and Cell Biology·Dongfang LiuEric O Long
Jun 17, 2010·The Journal of Biological Chemistry·Jimena Castillo BennettLuis S Mayorga
Oct 23, 2010·PloS One·Olivier MorteauChristopher A O'Callaghan
May 13, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto López-FloresLuis I Terrazas
Oct 29, 2008·Molecular Pharmacology·Gabriele Weitz-SchmidtSimone Riek
Jul 31, 2010·Journal of Leukocyte Biology·Tae-Jin KimKyung-Mi Lee

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