Calcineurin regulates phosphorylation status of transcription factor osterix

Biochemical and Biophysical Research Communications
Hirohiko OkamuraTatsuji Haneji

Abstract

Osterix is an osteoblast-specific transcriptional factor that is essential for osteoblast differentiation and bone formation. Calcineurin regulates bone formation through modulating osteoblast differentiation. However, post-translational modification of osterix such as phosphorylation and interactions between osterix and calcineurin remains unclear. In the present study, we demonstrated that calcineurin interacted with osterix determined by immunoprecipitation assay and Western analysis. Immunocytochemical study also revealed that osterix and calcineurin were co-localized in nucleus. Deletion of calcineurin binding motif on osterix molecule disrupted osterix-calcineurin interaction. Phosphorylation status of osterix was augmented by treatment with phosphatase inhibitors, FK506 and calyculin A. In contrast, treatment of recombinant calcineurin reduced phosphorylation status of osterix. Our present study suggests that calcineurin has an important role in the function of osterix through its modification of phosphorylation.

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Citations

Aug 5, 2010·The Journal of Biological Chemistry·María José OrtuñoFrancesc Ventura
Sep 2, 2011·Journal of Cellular Biochemistry·Kaya YoshidaHideo Yoshida
Dec 3, 2014·Molecular and Cellular Endocrinology·Sung Ho LeeKwang Youl Lee
Feb 14, 2013·Biochemical and Biophysical Research Communications·You Hee ChoiKwang-Youl Lee
Sep 4, 2020·Journal of Molecular and Cellular Cardiology·M D Jinqiang ZhuangJiang Hong

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