PMID: 2504804May 1, 1989Paper

Calcitriol inhibits the PHA-induced production of IL-2 and IFN-gamma and the proliferation of human peripheral blood leukocytes while enhancing the surface expression of HLA class II molecules

Journal of Endocrinological Investigation
G SaggeseA Toniolo

Abstract

1 alpha-dihydroxivitamin D3 [calcitriol; 1,25-(OH)2D3], the most biologically active metabolite of vitamin D3, exerts several effects on peripheral blood mononuclear cells (PBMC). We report here the effects of calcitriol on PBMC proliferation and on the expression of some lymphocyte surface differentiation markers, as well as its action on lymphokine production. Calcitriol inhibited the proliferation of PHA-activated PBMC in a dose-dependent manner, with peak activity at 10(-8) M. Exposure of PHA-stimulated PBMC to 10(-8) M calcitriol for 3 days tended to increase the percent of CD4- and CD8-positive cells, though statistical significance was not reached. A more striking effect of calcitriol was seen on the expression of the non-polymorphic determinants of HLA class II DR molecules; in cultures stimulated with PHA for 3 or 4 days; 10(-8) calcitriol doubled the percent of DR-positive cells as compared to controls treated with PHA alone. This activity peaked at 10(-9) M, a supra-physiologic dose. After 3 days in culture, 10(-8) M calcitriol strongly inhibited the production of both IL-2 and IFN-gamma. This effect was evident at different PHA concentrations (0.5, 1.5 and 3.0 micrograms/ml), and almost disappeared at 10(-10) M. The...Continue Reading

References

Mar 1, 1979·Annals of Internal Medicine·A A AbbasiA R Arnstein
Dec 1, 1987·Kidney International·M F Holick
Aug 1, 1986·The Journal of Clinical Endocrinology and Metabolism·S C ManolagasL J Deftos
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H ReichelA W Norman
Mar 15, 1985·Biochemical and Biophysical Research Communications·H P KoefflerA W Norman
Sep 16, 1983·Science·D M ProvvediniS C Manolagas
Oct 1, 1984·The Journal of Clinical Investigation·W F RigbyM W Fanger
Oct 15, 1981·Biochemical and Biophysical Research Communications·C MiyauraT Suda
Apr 1, 1985·Immunology Today·G Trinchieri, B Perussia
Jan 1, 1987·Immunology Today·K E SullivanB M Peterlin
Jan 1, 1988·Immunology Today·R W Baldwin

❮ Previous
Next ❯

Citations

Jul 30, 2014·Frontiers in Physiology·Thea K WöbkeDieter Steinhilber
Nov 25, 2003·Diabetes Care·Carolyn M FronczakJill M Norris
Mar 26, 2003·Annual Review of Nutrition·Matthew D GriffinRajiv Kumar
Nov 23, 2005·Pediatric Clinics of North America·Michael J HallerDesmond Schatz
Jan 13, 2015·Journal of Endocrinological Investigation·G BellastellaD Giugliano
Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Arndt VogelMichael P Manns
Sep 19, 2018·Journal of Pediatric Endocrinology & Metabolism : JPEM·Ioannis KyrgiosAssimina Galli-Tsinopoulou
Aug 22, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Jorge N ArtazaKeith C Norris
Dec 14, 2012·Expert Review of Respiratory Medicine·Joseph I Székely, Ágnes Pataki
Feb 22, 2017·Hormone Molecular Biology and Clinical Investigation·Simon Villegas-OspinaMaría T Rugeles
Mar 11, 2015·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Somnath BoseAlparslan Turan
Nov 10, 1998·European Journal of Immunology·M Cippitelli, A Santoni
Jan 27, 2005·The Journal of Nutrition·Susan S Harris
Feb 7, 2018·Antibodies·Andrew T LucasWilliam C Zamboni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.